How do we choose culture medium type; DMEM,MEM,RPMI?

Hello guys;I would like to learn how we can choose medium type. for example some papers say alpha-MEM is better than DMEM for human Mesenchymal Stem Cells (hMSCs) and some says DMEM is better than...

31 December 2014 2,163 26 View

Does anyone have any idea what would be the best way to mimic Extracellular Matrix for mammospheres?

For the stiffness and if it is possible also the proteins. I found one product called Matrigel (BD Bioscience) but it has fenol red.. I need something transparent. I would appreciate if any of...

01 February 2014 7,334 5 View

Cancer stem cells and nanomagnets

Could you suggest a paper that is about cancer stem cells and nanomagnets (nanocrystals..etc)?

06 July 2013 6,910 1 View

Cholera toxin

I am going to work with MCF-7 derived mammospheres. I need cholera toxin. Most companies sends in within 2-4 months. Any suggestion where I can get it? I am in Italy.

06 July 2013 480 2 View

Can anyone share a basic protocol for non-adherent mammosphere ( derived MCF-7) culture?

I want to have a non-adherent mammosphere ( derived MCF-7) culture. Can anyone share a basic protocol, not with bioreactors or complicated scaffolds =) Grazie

05 June 2013 3,011 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View