Can an original culture of 5-10 cells grow to be as large as 10 to the power of 12 cancer cells replicates in just 20 hours? Could this be correct or may his methods have been translated wrongly? See text between the * below, which is when this happens.
We isolated the malignant CTCs from the blood using Oncoquick with a membrane that isolates malignant cells from normal cells after centrifugation and positive selection using anti-EpCam and negative selection using anti-CD45 particles (isolated 3.2cells/ml, SD +/- 0.3cells).
*Then we developed cell cultures in a fetal calf serum media and at the same time we developed colony cultures in soft agar.* In each culture of the well plate we added a chemotherapeutic substance that is used in clinical application. Then we developed those cultures and we harvested a sample every 24 hours for 6 days and made the following assays etc.
Thanks for your help!
Hi Johanna,
There must be a typo or mistake in translation. Starting with 10 cells, and assuming a rather generous doubling time of 12 hours, you would only have 20,000 cells after six days! Where is this from?
Hi Steve,
Thanks so much for your reply. I think there was a mistake in the translation and that they're perhaps taking about nucleic acid amplification rather than cell culturing, perhaps.
Please have a look at this publication:
Article Abstract 1931: Tumor spheres growing from circulating tumor ...
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