Problems in silver staining of gels?

Hi, I have tried silver staining many times to stain gels, but i dont get the results. After incubating the gels in silver nitrate, i do not get any results.I have changed my silver nitrate too,...

03 April 2018 2,711 4 View

Chemically induced Glioblastoma Animal Model?

Hi, Can anyone advise me about chemically induced GBM model? How valid they are in human GBM research? Thanks

09 October 2017 7,722 1 View

Is there any tool/software etc through which I can search proteins based on its molecular weight?

Hi all, I have got some differentially expressed bands (proteins) on my SDS-PAGE, having molecular weight 40 and 35 kDa. Now I want to find out which proteins are they. Is there any software/tool...

07 August 2017 9,398 0 View

Quantity of proteins from frozen cell lines and fresh culture?

Hi all, I am extracting proteins from cells frozen at -80C. Will it effect the quantity of proteins as compared to the quantity extracted from fresh cell cultures? Thankyou

07 August 2017 8,014 3 View

Bioinformatics tools for proteins?

Hi all, I need to apply some bioinformatics tools on some novel proteins and publish it. Kindly help.

06 July 2017 9,585 3 View

Can anybody check the review I have written for publication?

Hi, I have written a review entitled: ABBERANT GLYCOSYLATION OF CELL ADHESION MOLECULES (CAMs) IN PROGRESSION OF CANCER: HIGHLIGHTING GLIOMAS. Can anybody check it for me before i go for its...

05 June 2017 646 1 View

Lectin Probed Western Blotting?

I need a protocol for Lectin probed western blotting for the analysis of differentially expressed glycoproteins.

31 December 2016 135 1 View

Protein Extraction from U87 MG cell lines?

Hi, Previously, I had done protein extraction from tissue (mice brian). Now I need to extract proteins from cell lines (U87 MG). Can i follow the same protocol? If someone has a protocol or...

31 December 2016 6,575 3 View

What is the best method for Staining and Destaining the gels in SDS-PAGE?

Hi, Someone please explain me the exact solutions which can stain and destain the gels after SDS-PAGE.

31 December 2016 5,241 11 View

Cell lines as control against Glioblastoma cell lines (U87)?

Hi, I am working on Glioblastoma cell lines (U87) and looking for aberrantly expressed glycosylated proteins. which cell lines can I use as control? Can I use normal mice brain tissues as control?

11 December 2016 4,572 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View