Can I generate Dendritic cells from NSG mice?

Hi I'm trying to generate murine DCs from NSG HLA A2 mice ,,, using 20ng GM-CSF However, all what I get is MHCII neg, F4/80 hi, which show they are macrophages ,,, So I wonder can I generate...

02 March 2019 9,233 4 View

Can I differentiate between mouse M1 and M2 Macrophages in FACs using these Abs only?

Hi every one I wonder if I can differentiate between M1 and M2 macrophages in mouse tumours by using the following markers only? CD11b, CD11c, Ly6C, Ly6G, F4/80, MHCII If not ,,,which Ab I...

05 June 2018 7,441 2 View

What is the difference between pleenocytes that are CD 45.2+ hi and intermediate?

Hi I'm doing FACs for spleens from C57Bl/6 mice and I find 2 populations : CD45.2 + hi and intermediate. What is the difference between them ? Thanks

05 June 2018 4,476 0 View

What are the best markers for immature Dendritic cells derived from mouse bone marrow?

Hi I need a good panel for my FACS experiment which I 'll differentiate (mature DCs- which pulsed with peptide) and immature DCs ? Thanks

04 May 2018 7,513 3 View

How many nM that I give to my mice according to the following info?

Hi I give my mice 15mg/kg of a specific drug So how many nanomolar I give to my mice If the drug's formula weight is 439.52g/Mol ?? Thanks

31 December 2017 6,581 3 View

How IFNg increase the expression of MHCI in vivo?

How IFNg can increase the expression of MHCI in vivo? what about in vitro? Thanks

03 April 2017 292 4 View

What is the X ray irradiation dose to stop proliferation of cancer cell lines?

I need to test the proliferation ability of anti-Her2 CAR T cells in response to Her2 expressing cell lines using 3H incorporation assay ,,, First I need to stop proliferation of my cancer cells...

03 April 2017 5,800 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View