Hi
What does the different shape of cells mean, invivo subcuatneous, cells are from single sorting
spindle shape and round shape of pancreatic cancer cell line from KPC mouse ?
thanks
If the cancer cells you are studying are secondary (metastatic), then its morphology (shape) may give you an indication about their origin (cell type in the primary tissue site of the cancer). HTH.
Hi I'm trying to generate murine DCs from NSG HLA A2 mice ,,, using 20ng GM-CSF However, all what I get is MHCII neg, F4/80 hi, which show they are macrophages ,,, So I wonder can I generate...
26 March 2019 1,186 3 View
Hi every one I wonder if I can differentiate between M1 and M2 macrophages in mouse tumours by using the following markers only? CD11b, CD11c, Ly6C, Ly6G, F4/80, MHCII If not ,,,which Ab I...
20 June 2018 7,317 2 View
Hi I need to infect dendritic cells derived from mouse bone marrow with adenovirus then co culture them with T cells I'm wondering how much time the dendritic cells need to process the virus...
05 May 2018 9,645 2 View
Hi I need a good panel for my FACS experiment which I 'll differentiate (mature DCs- which pulsed with peptide) and immature DCs ? Thanks
04 May 2018 2,145 3 View
Hi everyone I'm trying to titre some stocks of adenovirus that I isolated from infected 293a and I need a protocol to see the infection efficiency as the plaque forming assay However, I tried...
24 February 2018 7,073 3 View
Hi I give my mice 15mg/kg of a specific drug So how many nanomolar I give to my mice If the drug's formula weight is 439.52g/Mol ?? Thanks
27 January 2018 5,819 3 View
Hello everyone I wonder how can I pulse my mouse splenocytes with virus to be presented on MHC for co-culture experiments how many particles is enough for pulsing ,,, 10 virus / 1 cell? 50virus...
25 July 2017 4,223 2 View
Is there any difference when I find Monocytic or Granulocytic myeloid-derived suppressor cells ( MDSCs ) in the tumours? Thanks
17 April 2017 2,175 1 View
I need to test the proliferation ability of anti-Her2 CAR T cells in response to Her2 expressing cell lines using 3H incorporation assay ,,, First I need to stop proliferation of my cancer cells...
31 March 2017 7,268 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View