Is there any specific procedure / protocol to immunosuppress the mice and then to induce colon cancer using CT-26 cells? Can anyone suggest any specific immunosuppressors that can be used for inducing colon cancer?
I am calculation for optimization of TS of an aromatic molecule and a hydroxyl radical. In this DFT calculation, I am using the functional B3lyp/6-311+G(d,p) in the Gaussian16. The charge and...
04 April 2024 8,499 1 View
It is comsol 6.1 .
29 January 2024 3,626 1 View
Greeting everyone. Here, I can face a problem. I need to activate the legend section, but I cannot do this. Please help me. How can I activate the legend section? Here is the attached related file...
24 January 2024 185 1 View
Hello, During the simulation in comsol multiphysics I am facing the following error. Can somebody suggest me how to resolve this error.
18 January 2024 7,995 3 View
How the nozzle temperature can be increased without causing blockage or failure
21 August 2023 2,660 1 View
We wanted to create a syngeneic colon cancer mouse model for my research work. For this, I have chosen CT26 cells, a colon cancer cell line. Many literature reviews reported that the CT26 (1*106)...
20 August 2023 4,922 0 View
For the calculation of the total energy in VASP, it is recommended to use tetrahedron method with Blöchl corrections (ISMEAR=-5). And for 2D slabs in the z direction (perpendicular to the...
10 August 2023 6,615 2 View
I want to know the lock-in setting to measure impedance in three different frequencies. I configured three frequencies manually but didn't get enough output voltage for low frequencies, and the...
26 July 2023 4,771 0 View
We are making a topology file using the 'perl' command for Charmm2lammps conversion. We created master file by adding parameter file of lipid, carbohydrades, protein & cgenff. In that case,...
02 May 2023 1,951 0 View
When I have taken the cif file of "Sodium Vanadium Phosphate" for Na3 V2 ( P O4 )3, and when I read the poscar file in vesta or Medea VASP, it shows Na 24 atoms instead of Na 18 atoms [that is...
22 February 2023 2,351 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View