I have hydrolyzed my protein in 5 ml of 6N HCL and diluted the hydrolysate in 100 ml. Now, i have to run my sample on LC-MS. Therefore, i have to remove Cl ion from my samples before injecting it in LC-Ms. If i remove HCL through vacuum dryer then do i get rid off from HCL completely with no chlorine ion in residues?
or any other suggestion for running the samples in LC-MS hydrolyzed with 6N HCL?
Good afternoon! why would you remove the Cl ion? when evaporating, you will not remove them completely, this is not necessary, if there is a possibility of dissolving the desired analyte in organic solvents, then dissolve in it and when most likely you will not inject hydrochloric acid into MS. If there is no such possibility, then there is nothing to worry about that they will get into the MS, since in the process of hydrolysis the ions combine into organic salts, and the ion residues do not create a dangerous concentration of hydrochloric acid for the device.
Assuming the analysis target here is amino acids, after hydrolysis to get rid of Cl and other interferences, it is advisable to perform a cation exchange treatment. Protonated amino acids will retain on resin while most of the neutral and anionic species flow through. After elution, you can analyze AAs either by HILIC or derivatization followed by RP chromatography...chloride removal by forming CuCl, AgCl, BiOCl, and Friedel's salt (complexation-induced precipitation) is an alternative strategy but not necessary for this case...
I suspect that you cannot totally get rid of all the chloride - some minute amounts will be bound to any positively charged entities in your sample. Try redissolving in your sample in dilute formic acid and then redry it. Small amounts of formic acid will not affect LC-MS.
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