Lentiviral aggregation in sucrose solution?

18 August 2023 0 8K Report

My team mates and me have a problem with a lentiviral aggregation. We don't know how sucrose could impact in the aggregation explained. Could anyone solve this question please?

Badges
Science method

More Jorge Sangüesa's questions See All

How to Input and Analyze Results for an Asymmetrical Factorial Design in STATISTICA 13.3?

In STATISTICA 13.3, I used the Experimental Design Builder under the Design of Experiments tab to generate a design matrix for an asymmetrical factorial design (1443) with 12 experiments. However,...

29 July 2024 4,155 2 View

What is the relationship between Artificial Intelligence and Communications?

Much is said about AI and the Communication field. Some say that Communications would very much benefit from that intersection. But specifically which areas would benefit from AI? Could it be a...

11 July 2024 5,335 4 View

Protein degradation? how to concentrate low MW proteins?

Hi everyone! So I am trying to concentrate my recombinant proteins in order to make some assays with them, but I need a "considerable" concentration of each. One of my proteins is around 9kDa and...

08 May 2024 5,418 2 View

How reliable is Fiji software for analysis of movement organisms?

Dear all, My question is addresses to reliability regards how reviewers or journal editors would take it when they realize that the methodology of a paper was mainly done through this software. I...

15 April 2024 9,305 0 View

What is the refractive index of MoSi2?

Does anyone know what is the refractive index of MoSi2 and in what medium it can be performed (distilled water or ethyl alcohol)? I pretend to perform a particle-size distribution analysis by...

23 March 2024 6,736 1 View

Where to order research-grade Galleria mellonella?

Does anyone know where to purchase research-grade Galleria mellonella in Europe? I only find the TruLarv from BioSystems Technology, but it seems to be down... Thank you!

11 March 2024 4,800 2 View

Could someone recommend me a valid instrument to measure teaching performance that utilizes AI (Preferably focused on elementary school teachers)?

I am conducting research on the performance of primary school teachers who use AI, and I have not been able to find a validated instrument that fits this situation. I would like to hear your...

22 February 2024 6,052 4 View

Sauropod-like vertebrate teeth?

Is is possible that Cretaceous fish, notosuchians, lepidosaurs, or other vertebrates have a morphology that might be confused with "spoon-shaped" sauropod teeth? I have seen a fossil isolated...

08 December 2023 7,040 2 View

Could someone provide me with two JCPDS cards?

I would need two JCPDS cards: 1. BaO (No. 26-0178) 2. Ba(OH)2 (No. 026-0154) I would appreciate a lot if someone could send me both cards... I am a bit desperate. Thanks again for your time!

08 October 2023 4,324 4 View

Is there an accepted taxonomy or categorization of environmental issues (e.g., waste management, water pollution, etc.)?

I am currently working on enhancing the H4rmony dataset, which aims to fine-tune Large Language Models in AI to align them with ecolinguistic values. I'm seeking this classification to assist in...

27 September 2023 972 3 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

Franklin M. Fisher (1983) Disequilibrium Foundations of Equilibrium Economics. How is it estimated now? How is it related SMD results?

Fisher studied if there is an out-of-equilibrium process that rapidly converges to some equilibrium points. He claims that Hahn process has a Lyapounof function and therefore convergent to an...

15 July 2024 1,460 4 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

Why am I seeing only one initial maximum in torque-time curves for the fusion of PVC in a Brabender?

We're studying the stability and fusion behavior of multiple PVC compounds in a Brabender. After a few trials, we set the temperature at 195 °C and the rotor speed at 70 rpm. Samples differ in...

12 July 2024 2,768 1 View

Stable cell line generation: GOI is detected by qPCR but protein is not expressed?

Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...

11 July 2024 4,812 5 View

I have constucted a plasmid vector from puc19 with no antibiotic and lacz gene. The problem is screening for transformed cells. Any suggestions?

Get too many colonies, which are mostly non-transformants when tested by colony PCR

11 July 2024 6,775 3 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to see whether my protein is aggregated using western blotting?

I am working on one protein which gets aggregated (similar to alpha-synuclein and tau) in certain neurodegenerative diseases. I am trying to confirm the aggregation using Western blotting....

30 June 2024 5,060 3 View

Self discharge of freshly made NMC half cells and voltage stabilizes at 3.3-3.5 V vs Li/Li+ during charging. What can affect it?

I am making Li half cells using NMC as cathode. The OCV is around 3 V but it self discharges and during charging the voltage increases to 3.5 V and then drops to 3.3-3.4 V and stays there. I have...

23 June 2024 842 3 View