I have been using this isolation kit for a long time and I am now just noticing that my yield has been progressively reducing.
Write an essay on the same
28 June 2024 3,004 3 View
Write an essay
28 June 2024 2,537 3 View
Is there an alternative approach to incorporate research methods into a book chapter without dedicating a separate section for methods? If so, how can this be achieved, and where within the...
06 April 2024 7,900 3 View
Hello. I was trying to simulate the hydrated Nickel cation [Ni(H2O)6]2+ and my calculation setup is not working. For that, I used the aug-cc-pVDZ basis sets for H and O; and Lanl2DZ for Ni....
25 March 2024 1,614 4 View
I'm trying to simulate the sulfate anion structure in Gaussian, but my double bonds disappeared after the calculations. I had the same problem when I was trying to simulate the acetate anion....
04 March 2024 488 3 View
Research data early childhood trauma and addiction
02 March 2024 6,731 2 View
I'm trying to simulate the complex [V(H2O)6]3+ using Guassian, but the calculation does not converge.
24 February 2024 5,098 2 View
In some countries (e.g., Italy), there is an obligation for hotels of certain categories (4/5 stars) to equip guest rooms with safety containers/in-room safes. However, we wonder whether a prior...
16 January 2024 1,436 5 View
I would like to publish an article on the MSc Thesis I am concluding.
24 September 2023 8,311 1 View
I am standardizing a UV-VIS screening method that allows me to quantify provitamin A carotenoids. The results will be compared with samples quantified by HPLC, which is my Gold Standard....
06 September 2023 1,850 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...
31 July 2024 6,464 5 View
I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...
28 July 2024 7,827 6 View
Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...
26 July 2024 5,654 3 View
I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...
24 July 2024 9,799 3 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...
16 July 2024 7,475 1 View