Could anyone help me to perform EMSA for Pu22 oligonucleotide with a small molecule?? The bound DNA and unbound DNA was not observed when we performed the assay in TBE buffer with voltage 70-100V and Acrylamide GEL % 8-25.
I appreciate your time and help.
Thank You
It sounds like you're trying to perform an Electrophoretic Mobility Shift Assay (EMSA) with a Pu22 oligonucleotide and a small molecule, but you're not getting the expected results. There could be several factors affecting the outcome, such as the binding conditions, buffer composition, and gel conditions. You might want to consider adjusting the binding reaction conditions, optimizing the gel percentage, and ensuring proper sample preparation. Additionally, checking the purity of your Pu22 oligonucleotide and the small molecule could be important. If the issue persists, consulting with a colleague or expert in the field could provide valuable insights.
- Badges
- Science method