Is there any method that we can electrochemically oxidize a powder?

I am working with a material which is sensible to water and oxygen, I would like to oxidize this powder either chemical or electromagnetically. Does anyone knows a method to electrochemically...

09 February 2021 2,944 3 View

How to make sure each cavity modes with same E-field distribution in COMSOL simulation?

In COMSOL Application Library, there is case about waveguide adapter. But there are some questions: 1) In boundary mode analysis, it just set to find only 1 mode and the S-parameter is...

12 January 2021 9,383 2 View

Generate a structure file through PGDSpider?

Hi, I am trying to generate a structure file through PGDSpider, for a diploid organism, where each locus is in two consecutive columns in one row, rather than data for each individual be stored...

29 December 2020 5,169 1 View

Why the treatment days for the xenograft model is less than the observation days?

In some experiments for investigate drug effects for in vivo xenograft model, some authors onle give treatment to the mice for 14 days or 21 days, but the data collection/observation (such as...

13 December 2020 525 2 View

Can distributed reinforcement learning realize distributed learning process?

The current distributed reinforcement learning framework, such as IMPALA, uses the structure of centralized learning and distributed execution. I feel that this is not really distributed. Is there...

02 December 2020 5,598 4 View

Is there any possible stabilize metal-doped blue-emitting light perovskite through self-assembly method?

Now, I have got a metal-doped perovskite in which blue shift happens result. I tried to make it a more blue shift to get a stable and very blue perovskite. So I think is there any possible...

04 November 2020 8,384 3 View

Why perovskite thin film PLQY much higher than dispersion PLQY?

I doped Mn into perovskite. I found when up to the best doping amount, the thin film PLQY much higher than the dispersion PLQY in my experiment. I don't know what caused it, is there any theory...

04 November 2020 2,477 3 View

Is there any relationship between crystallinity and PL indensity of perovskite?

I added the Mn dopant into the MAPbBr3 perovskite. When Mn dopant I added up to a certain amount, pl intensity can get a high result, but in the next day, the highest result I got decreased...

05 October 2020 9,192 7 View

How do I change to make an insoluble recombinant protein to a soluble protein?

How do I change insoluble recombinant proteins to soluble proteins? My recombinant protein is Aldose dehydrogenase from bacterial with around 35.0 kDa size. Now, My expression vector and...

05 October 2020 1,840 2 View

Why PLQY of perovskite can decrease too much the next day?

I tried to use Mn dopant into MAPbBr3 perovskite, but,I don't understand why the plqy of the thin film will decrease too much in the next day and then after several days it can also get increased...

04 October 2020 3,464 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View