Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Does anyone know the neutrophil marker for qPCR in human gut (duodenum, ileum, colon) biopsies?

Hello everyone. I have collected intestinal biopsies from stem cell transplanted patients in RNAlater and wish to quantify neutrophils only via qPCR. As we know that the mucosa of gut is filled...

25 November 2020 2,680 3 View

RNA extraction mouse air pouch?

Hello, We are currently working on an inflammatory model using a mouse air pouch and we are having some trouble with evaluating gene expression using this tissue. We have been using the Qiagen...

22 November 2020 1,040 1 View

Significant degradation of RNA in RNAlater storage at 4C for longer than a month?

I misread the amount of time that RNA was stable in RNA later at 4 C as several months instead of a month. I have e zebrafish samples that have taken me a 2 months to collect and they have been...

23 October 2020 8,085 3 View

Is RNALater (Qiagen) or Nucleoprotect RNA (Macherey-Nagel) compatible with High Pure Viral Nucleic Acid Extraction Kit?

I am planning to preserve fresh bat guano in either RNALater or Nucleoprotect RNA but my lab has an excess of High Pure Viral Nucleic Acid Extraction Kits. Any idea if both reagents are compatible...

14 October 2020 2,848 2 View

Am I introducing any potential loss of RNA integrity by combining an RNALater solution with a non-ionic detergent?

I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...

10 October 2020 1,830 3 View

What could be the problem with my RNA extraction using RNeasy Mini KIt from Qiagen ?

Dear Researchers, I am going to run RNA Sequencing on mouse brain samples. I have collected the samples and then I put them in the RNALATER solution and stored them at -80. Following this...

06 October 2020 6,439 3 View

Anyone with experience on DNA extraction from biopsies for microbiome analysis?

We shall collect biopsies from skin/hoof of animals for bacteriological microbiome analysis. We need suggestions on DNA extraction kit that may be used for this. We prefer to keep the biopsies in...

24 September 2020 6,845 4 View

What is the best way to keep tissue samples in during dissection for ELISA (besides snapfreezing in LN2)?

Hi, I wonder if there is any buffer that i can keep tissues in, right after the dissection (mice). That would be very handy because there will be a lot of sample collection going on during...

12 August 2020 8,176 3 View

Pros/cons of washing tissue in PBS prior to snap freezing?

Does anyone have protocol recommendations for freezing/storing human uterine tissues? I have seen some protocols that place tissues directly in LN2 and then transfer to -80 for long term storage....

02 March 2020 3,393 2 View

Any recomendations for selective lysis of Eukaryotic cells stored in RNAlater or Ethanol?

Hello everybody, --just a brief intro about the background of my question-- I have to analyse the gut microbiome of a marine fish through whole metagenome sequencing. Going through literature, I...

18 February 2020 8,460 1 View