Dear colleagues,I have obtained promising experimental results showing synergistic antibacterial effects between colistin and plant species polyphenolic extracts, both in vitro and in vivo. The combination demonstrates:

• Enhanced bactericidal activity against multidrug-resistant Gram-negative bacteria
• Reduced colistin-induced nephrotoxicity
• Significant reduction in minimum inhibitory concentrations

I am now looking to understand the molecular mechanisms underlying this synergy through in silico approaches. Specifically, I need help with:

• Colistin and bacterial membrane lipopolysaccharides
• L. spathulatum polyphenols and potential targets
• Analysis of potential interaction sites

• Membrane destabilization mechanisms
• Potential interactions between compounds
• Structural changes in bacterial membrane

Any suggestions for appropriate software, methodologies, or collaboration opportunities would be greatly appreciated.Thank you in advance for your guidance.Best regards,