Hi. I am currently doing a project regarding the measurement of TPC using FC reagent. My results indicated that the TPC for hexane fractionated extract was quite low, and I am just curious on how to I present it. Can anyone share related reference paper regarding this?
Shiwa Chaubey
- Quantitative Expression:Report the TPC values in standard units, such as milligrams of gallic acid equivalents per gram of extract (mg GAE/g extract). This standardization facilitates comparison with other studies.
- Comparison with Other Fractions:If your study involves multiple solvent fractions, present a comparative analysis. For instance, display the TPC values of the hexane fraction alongside those of other fractions like methanol or ethyl acetate. This comparison can highlight the distribution of phenolic compounds across different solvents.
- Graphical Representation:Utilize bar graphs or scatter plots to visually represent the TPC values of various fractions. Visual aids can effectively convey differences and trends in your data.
- Discussion of Results:Interpret the low TPC in the hexane fraction by considering the solvent's polarity. Hexane is non-polar and may not efficiently extract polar phenolic compounds, leading to lower TPC values. Discuss this in the context of solvent selection and extraction efficiency. Pérez, M., Dominguez-López, I., & Lamuela-Raventós, R. M. (2023). The Chemistry Behind the Folin-Ciocalteu Method for the Estimation of (Poly)phenol Content in Food: Total Phenolic Intake in a Mediterranean Dietary Pattern. Journal of agricultural and food chemistry, 71(46), 17543–17553. https://doi.org/10.1021/acs.jafc.3c04022
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