Techniques for counting bands and alleles
Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...
03 August 2024 2,546 2 View
I was following this chip-seq protocol https://www.cellsignal.com/products/chip-kits-reagents/plus-sonication-chromatin-ip-kit/56383 what will be the consequence if on the following state "VI....
20 July 2024 5,612 0 View
Any Universities which accept students to PHD program after a undergraduate degree.
06 July 2024 3,903 2 View
I am trying to do a matlab project on power system state estimation. I am doing using WLS,EKF and particle filter method and then comparing these all. To minimize the error can we use Machine...
04 July 2024 6,845 0 View
I randomly interviewed 250 poor people and 250 non-poor people. Considering 1 for poor and 0 otherwise, does estimating a logit model aiming to capture the probability of becoming poor make sense?...
02 June 2024 2,326 2 View
I would be grateful if anyone could provide the answer to these question. I am using discovery hybrid rheometer HR-3. Thanks a lot
31 January 2024 4,402 0 View
I was hoping to find yield stress value using Carson or Herschel bulk key model.. I tried to fit viscosity vs shear rate plots to these models. Howeve, I can’t select these two model in our...
31 January 2024 6,318 0 View
I am trying to evaluate the genetic homogeneity of wild grown mother plant and tissue culture raised plants of Piper sp. using SCoT and other dominant markers.
06 December 2023 1,837 0 View
I have plots of shear stress vs shear rate and viscosity vs shear rate. I want to check which rheological model do my systems (hydrogels) follow and calculate the parameters form the best fitting...
14 November 2023 6,377 7 View
I am currently studying in Nepal
26 October 2023 639 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.
02 August 2024 6,789 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...
29 July 2024 2,229 0 View