Can anyone suggest me a good source to find LCI of smart box/energy box use in PV off-grid power system ?

Smartbox or energy is an important item of a solar power system. I would be grateful if someone can help me/suggest a literature source to find life cycle inventory of the smart box. Thanks.

25 November 2020 1,542 1 View

Is it possible to get a small heater 30*30 mm size, which can work up to 200 degrees C?

Is it possible to get a small heater 30*30 mm size, which can work up to 200 degrees C? As part of my research, I need a small heater 30*30 mm size with the thermostat, which can work up to 200...

15 September 2020 8,632 3 View

Do we always need to have error degree of freedom greater than 12 to start our research ?

I have attended different universities during my study programme and for different conference and seminars, where I notice a critical number always suggested by almost every scientist/professors...

03 December 2019 420 3 View

Climate change is real. Is it really going to have some significant negative impacts on agricultural output ?

Climate change is real and over the years the weather parameters are changing, so the global hydrological cycle, atmospheric circulation, bio-geochemical cycles also changing. But to some extent...

25 November 2019 9,336 8 View

No title

I am using iron oxide nanoparticles for metal adsorption studies and observed that two different particles (two colors and sizes) existing. the manufacturer sells them as magnetite nanopowder. I...

14 March 2018 9,362 8 View

Does anyone know of literature on NSAIDs information for breast cancer cell line?

12 November 2015 1,285 2 View

What is the best method to isolate viable single cells from fresh adult mouse/rat brain?

I'm trying to isolate viable single cell suspension from brains harvested from 2 month old mice/rat. I'm specifically looking for enzymatic methods of dissociation. I understand pappain works...

18 August 2014 4,633 4 View

Can anyone help with the quantification of interferon alpha and interferon beta?

I would like to quantify IFN-alpha and beta from cell culture of whole blood/mononuclear cells after stimulation with LPS or other stimulants. Can anyone suggest me the conditions of cell culture,...

06 March 2014 1,216 2 View

How to calculate specific heat from MT data of a magnetic material?

Applying clausius - clapeyron equation?

04 March 2014 6,879 2 View

Which plates should I use for AlamarBlue assay?

Cell viability assay

30 July 2013 4,837 8 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View