Other electron transfer mediators of phenazine methosulfate (PMS) in glutamate dehydrogenase (GDH) isoenzyme research are some chemicals with similar redox properties but with better stability and lower toxicity. Meldola Blue (MB), a phenoxazine dye, has very good abilities to transfer electrons between reduced nicotinamide adenine dinucleotide (NADH) and terminal electron acceptors and has better stability under laboratory conditions. Phenazine ethosulfate (PES) is analogous to PMS and possesses the same electron transfer capability. The 1-methoxy-5-methylphenazinium methyl sulfate compound can withstand degradation by light more. Enzymes such as diaphorase can facilitate the transfer of electrons from NADH to other tetrazolium salts (such as WST-1, WST-8, and MTT), which allows us to visualize the activity of GDH through color change. Also, 2,6-dichlorophenolindophenol (DCPIP) and potassium ferricyanide are electron acceptors that don't require intermediate electron carriers at all. When selecting, you must consider redox potentials, reaction velocity, light characteristics, and compatibility with methods that measure electrical charges or detect light to ensure we can observe the GDH isoenzyme patterns well, as with usual PMS-based assays.

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