I want to use CAS9 systems to knock in expression cassette into CHO cells to make stable cell lnes. how do I choose the hot spot for knocking in?

09 September 2014 1 7K Report

Cas9 is a good genome editing tool. I want to make stable cell lines using CAS9 knocking in tech. I don't know how to choose hot spot for inserting. Does anybody know about it?

Subin R. C. K. Rajendran

You should refer to the safe harbor loci in the human genome.to which, the transgene should be preferable targeted.

Article Functional genomics, proteomics, and regulatory DNA analysis...

Article Safe harbours for the integration of new DNA in the human genome

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