I am going to optimize the medium composition for enzyme production. there are many optimisation method such as RSM, Taguchi Orthogonal array etc... Which method is best for medium optimisation?
Hi,
we are using genetic algorithms for medium optimisation. Just search for these keywords in google scholar and you will find a huge amount of literature. From what I know from other methods the use of genetic algorithms needs less manpower to succeed.
Hi,
I would suggest you to go for some statistical optimization methods for enzyme optimization. Response Surface Methodology (RSM) is a statistical method which operates on different mathematical models. this would definitely reduce your time and will optimize the medium for enzyme production
I would suggest Response Surface Methodology (RSM). You can use SAS software or Design Expert to do the data analysis.
There are different methods of the experiments optimization. We use the orthogonal rechtangulars method..
hi
I am trying the medium optimisation by Taguchi orthogonal array using Design expert software version 7. Finally i didnt get any F value but there is a column for it............. how to rectify it......... can anyone guide me..........
PROGRAMMING OF EXPERIMENTS FOR THE OPTIMIZATION OF COMPLEX PROCESSES BY MEANS OF ORTHOGONAL LATIN SQUARES
Biryukov V.V.
Pharmaceutical Chemistry Journal. 1968. Т. 2. № 1. С. 51-55.
In this paper the many-level method of experiment design is described/
You follow this route for media optimization:
One factor at a time -> Plackett-Burman Design -> Response Surface Methdology -> Genetic Algorithm -> Artificial Neural Network.
Yes, the statistical methods. But there are a lot of them. Try the simplest!
Use RSM based media optimization.
1. You can use Plackett–Burman design to screen the factors influencing your enzyme production.
2. The effective concentration of the selected factors can be calculated with the help of Central Composite Design
You can use Minitab or Design expert statistical software
I suppose that the enzymes biosynthesis better to perform in two stages. First is obtaining enough biomass with fermentation media optimized for growth rate. The second is a feed batch stage in which one or two substrates added continuously in order to maintain their optimal for enzyme biosynthesis concentrations in fermentanion liquid.
Best regards!
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