Do you know a gen or genes that are suitable reference genes for quantitative gene expression analysis by real-time RT-PCR in Aspergillus niger?

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Maria Cristina Rocha Cordeiro

Dear colleague,

The best reference genes for qPCR must be studired first for each biological systems. They must have similar expression level concerning different tissues, stages of development and environmental conditions. It must be the first part of your expression analysis job. Good luck!

Aarón Barraza

I suggest to design primers to amplify the 16S rRNA to be used as a reference gene in Aspergillus niger for qPCR expression analysis.

Regards