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I want to learn how to use NCBI stand alone application but it seems am making a mistake when making a database Have attached the response i keep getting (C:\Users\Edwin...
14 June 2022 4,985 4 View
Good morning. I am trying to obtain all the Mpro variant's sequences from Sars-Cov2 I understand I need to use NCBI, but I do need a more detailed explanation of how to obtain what I need. I have...
10 June 2022 7,739 0 View
Hello I am trying to find a website or freeware that predicts amphipathic helices from AA sequence. I have been trying AMPHIPASEEK (AMPHIPATHIC IN-PLANE MEMBRANE ANCHORS PREDICTION),...
08 June 2022 5,460 1 View
Could anyone help me with the following question? I was trying to purify my protein of interest by immunoprecipitation (IP) and look at the phosphorylation status of the protein using specific...
28 April 2022 5,448 2 View
What colour should PichiaPink be on a rich media that has adenine in it? From what I can tell, the presence of adenine represses the whole pathway of adenine synthesis in PichiaPink, meaning the...
28 March 2022 3,934 0 View
Dear all I would like to model a miRNA-mRNA duplex 3D in pdb format using prediction tools. I have used viennaRNA package by RNAfold using the following command "$ RNAcofold -p --noGU
06 March 2022 7,929 0 View
I knocked down a gene in human neural progenitor derived neurons for a neurosphere migration assay. I found that the KD neurons migrated longer distance than WT neurons. However, when I knockout...
04 March 2022 8,802 0 View
I'm currently working on a GPCR and I need to model its N-terminal residues which are missing in its PDB structure. I need to have the correctly modeled N-terminus because I need to check its...
04 March 2022 3,043 4 View
Hi, I have just started using snapgene and have downloaded a plasmid sequence off addgene to have a play with. Addgene says a gene is under control of a KasOp promoter, but when I insert the...
11 February 2022 1,406 1 View
Systems biology is a discipline to understand not how an individual’s function but how complexity systems (many interacting parts) in biology works. It Involves very precise measurement of...
07 February 2022 5,684 3 View
Many research articles have removed the 3rd codon positions in PCGs and rRNA genes for the Phylogenetic tree construction.
03 February 2022 3,872 2 View
Hi there, I want to analyze deferentially gene expression of mice before and after treatment. I have 6 mice and "paired-end" sequences, so how I could merge all my "before treatment" data to...
22 January 2022 3,648 6 View
Hello everybody, Recently we faced a problem with emerging red leaf spots on fiddle-leaf fig (Ficus lyrata) plants (as shown in the attached picture) under the greenhouse conditions (25 °C, 50%...
21 January 2022 1,669 7 View
I recently did a western blot of a secretory protein, the result shows that the size of this protein in cell lysate is smaller then it in culture supernatant. Is this normal? Do some people have...
24 December 2021 7,475 5 View
I am looking for good pollen substitute to feed honeybees during dearth periods
22 December 2021 3,434 3 View
Hi everyone, I want to prepare a sodium phosphate buffer of 0.02 M (pH 7 and 7.8). Could you help me, please? I want to prepare this solution by using di-sodium hydrogen phosphate dihydrate...
11 December 2021 9,245 3 View
Greetings. Several authors have identified in the past what seems to be a bacteriophage-derived antivirus against certain eukaryotic viruses, although no recent literature seem to cover similar...
07 December 2021 5,798 3 View
Glucose standard preparation for DNS assay
27 October 2021 7,209 3 View
We used DNeasy® PowerSoil® Pro Kit (QIAGEN) to extract DNA from soil. However, the yeilds was very low and the concentration was at about 10ng/ul although we processed totally following the...
24 October 2021 3,062 0 View
Missing residues in Schrödinger's Prime homology model.
19 October 2021 5,023 3 View
I am using three different multiple sequence alignment methods which are clustal omega, Mafft, and muscle. On sample of data , the length of generated sequences after alignment was on equal (same...
07 October 2021 8,474 1 View
There are many visualisation software and their mechanism are more different from each other. The visualisation technique is important for my research.
29 September 2021 3,250 13 View
Can anyone help me with protocol for removing host DNA contamination from phage lysate. Any protocol by using silica gel matrix for removal of host DNA
24 September 2021 1,860 4 View
I need to check the ligand-receptor interaction on the Discovery studio analyzer or another software but I have separate PDB files. I tried to merge the ligand (which is in a protein format) and...
14 September 2021 8,322 6 View