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Questions related to Bioinformatic Tools
Hi all, I´ve found some weird structures in my cell culture flask. They seem to be under the cells or sticking to the plate, not floating. I have seen mold contamination before, and it doesn´t...
29 October 2024 3,357 6 View
I want to know about the different benefits of mangroves.
26 October 2024 5,466 3 View
Salinity causes stunted growth of mangroves
26 October 2024 7,418 3 View
HI all, I have started cell culture for VSMC and I saw this in my culture the other day. It does not move when gently jiggling the plate and I have seen it even after rinsing with PBS and...
25 October 2024 4,501 2 View
I've been using the mFD package to calculate both Alpha and Beta functional diversity accounting only for the presence and absence of species, but I need to include their abundance at least for...
24 October 2024 762 5 View
We are going to study some MOFs, but I have no experience at all. I did some calculations using GROMACS, so now I have the following questions: - as I understand, it is possible to evaluate the...
04 October 2024 2,181 2 View
While perusing the literature, I observed that some researchers perform NVT-NPT-Production runs to study quantum dots with classical MD. However, many studies have also examined where...
02 October 2024 9,124 1 View
I wanted to do a protein and ligand simulation in GROMACS when I encountered this error, and my force field is CHARMM: The residues in the chain THR32–HIS369 do not have a consistent type. The...
17 September 2024 353 1 View
I have LCMS raw data how should I know which glycoprotein is upregulated and which one is downregulated? Through MALDI-MS data how to identify glycopeptides? I have used HILIC material to enrich...
11 September 2024 7,398 0 View
I am currently working with the Panc1 cell line and have observed some black dots or thread-like structures in my culture. These structures do not seem to interfere with the growth of my cells....
06 September 2024 9,622 1 View
I need to geometry optimization of the Ca complex in orca 4 2 1 by using mixed basis set (6-31+g(d,p)/LANL2DZ). if known the input file is attached.
02 September 2024 8,482 1 View
Sonication: I'm currently optimizing the sonication conditions for shearing chromatin in HepG2 cells using the Bioruptor® Pico for downstream applications like ChIP-qPCR. While I've tried various...
18 August 2024 5,054 0 View
Technology is fast developing. It has left us with no option than to accept, adopt, and enjoy it, while it lasts. Teaching and learning experiences can be fun for both teachers and students if...
10 August 2024 10,003 4 View
Some has the crack version of this tools?
24 July 2024 1,419 0 View
Hello, I recently made a fresh stock, filtered it, and then incubated it for a sterility check. My media is still pinkish but it has some whitish floating dots over it. This is how they looked...
23 July 2024 8,229 1 View
there are tiny movable dots and rods like strucrture and there are filled in my cell plate. i have no alternative but to eradicate them from the cell as I have no other stocks left. so How can I...
01 July 2024 4,767 0 View
I want to compare "Dont Eat Me" markers on M1 and M2 macrophages. We have commercial media available for differentiating human PBMC-Macrophages and than differentiate them to M1 and M2. Do we have...
18 June 2024 4,737 0 View
Hi I need to simulate a phosphorylated protein. I already have found information of how to introduce a phosphorylated residue in gromacs...
13 June 2024 1,961 0 View
In Iran, there is informal co-creation of curriculum in higher education; But formally, no What do we need to implement curriculum co-creation in higher education?
29 May 2024 3,181 14 View
Are there any specific considerations for the dosing and administration of dexmedetomidine in patients with renal or hepatic impairment?Dexmedetomidine: A Comprehensive Review of Pharmacology,...
09 May 2024 1,784 1 View
I found some self-assessment models of information literacy in the literature. However, I would like to know if there is any model or tool that assesses knowledge and skills related to information...
22 March 2024 4,172 2 View
I am interested in the study of dendritic cells response under infection with mutant strain candidate to vaccine against TB. But I am begginer in the work with dendritic cells, somebody can help...
21 March 2024 1,653 7 View
I have designed 2 primers Now I need to set up the PCR protocol. First: I need to know how much of each primer, H20 and GoTaq® G2 Hot Start Taq Polymerase to put in the mix. We usually put a...
29 February 2024 294 2 View
During preparation the sample, I want explanation on what is the meaning of doing a triplicate tube from the sample, is that meaning prepare 3 tubes from the single sample or doing a serial...
20 February 2024 5,433 2 View