Hi everyone, I want to prepare a sodium phosphate buffer of 0.02 M (pH 7 and 7.8). Could you help me, please? I want to prepare this solution by using di-sodium hydrogen phosphate dihydrate (177.99 g/mol) and Sodium dihydrogen phosphate dihydrate (156.01g/mol).
The experimental method:
Prepare 20 mM solutions of each.
Calibrate a pH meter with pH 7 and pH 10 standards.
Get a magnetic stir plate, stir bar and a beaker.
Half fill the beaker with the the Na2HPO4 solution.
Gradually add the NaH2PO4 solution while stirring and measuring the pH.
Stop when the pH reaches 7.8. This is a 20 mM solution, since both of the original solutions were 20 mM.
Or, continue until the pH reaches 7.0.
You can use the buffer solution calculator of sigma-Aldrich to easily calculate the buffercomposition:
https://www.sigmaaldrich.com/TR/en/support/calculators-and-apps/buffer-calculator
You may want to check this related RG query:
https://www.researchgate.net/post/Why_do_I_struggle_to_adjust_lower_pH_of_my_phosphate_buffer_at_a_certain_pH_point_even_though_I_add_too_much_acidic_solution
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