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Questions related to Bioinformatic Tools
What should I do next when I get a sample of APEX2-MS (Proximity-Labeling MS)? Western blot or Silver Stain?
13 February 2025 398 0 View
I’m particularly interested in: Strategies to select variable regions (e.g., V3-V4 vs. V4-V5) for broad taxonomic coverage. Tools/software for in silico validation of primer specificity and...
10 February 2025 2,878 3 View
Dear friends, I am looking for the ionization energy of triphenyl phosphate, C18H15O4P. I also want to confirm whether the excitation energy, UV/Vis spectrum, is Eg = 4.6 ± 0.1 eV?...
04 February 2025 8,618 0 View
i am a student i need papers for my research paper but i cant find it so help me my topic and a view The Medicine Assist app is an innovative digital healthcare tool designed to optimize...
03 February 2025 8,795 0 View
My study is about cell metabolomics. I’m currently analyzing MS/MS data using MS-DIAL 5 for untargeted metabolomics, but I’m facing an issue where I don’t get any...
03 February 2025 3,678 2 View
Dear colleagues, Im looking for Q1, Q2,Q3 digital health journals indexed in Scopus without fees,without APC.
02 February 2025 7,185 2 View
Hi, We are going to perform a ChIP-seq assay and need to fragment our chromatin to obtain fragments of between 200 and 1000 base pairs. In each of our samples we start from 5-10^6 cells. We will...
02 February 2025 10,027 0 View
Why Do Most Clinicians Support the “One-phase Treatment”?
29 January 2025 9,645 3 View
Hi all, I'm trying to use AutoDock Flexible Receptor (ADFR) to do a flexible docking between a membrane protein and its ligand. I already got some candidate poses, and I'd like to validate them...
22 January 2025 1,340 3 View
Peltz and Südhof (2018) noted that many opioid users have several changes in their brains, hence the need to investigate the molecular signals that cause these changes. Reference the citation
21 January 2025 597 0 View
I have uploaded docked complex of two proteins in pdbsum server. Both the proteins have different chain names in the pdb file. However, the pdbsum generated file is not showing protein-protein...
08 January 2025 2,309 0 View
Hi, I am doing the culture PBMCs from healthy humans in which B cells are stimulated by anti-Ig and TLR7 ligand. Very oddly, on day2, I see dramatic decrease in the ratio of B cells compared to...
08 January 2025 9,204 0 View
Dear searcher, I have a problem in anti-inflammatory assay using albumin denaturation,as soon as I mix the plant extract in DMSO solvant with albumin solution (human albumine 20%) there is the...
07 January 2025 2,225 2 View
Hi, I am looking at MaxQuant (MQ) quantification of protein ids across several samples. I am using unique + razor peptides, which is provided as a list in proteinGroups.txt, then summing the...
01 January 2025 4,433 1 View
I want to dock the glutathione to a protein on a specific site (for the site,i have identified with MS), how can i set the paramater of the AGFR, should i still need to compute pockets?
12 December 2024 6,583 0 View
Hello! We are experiencing an issue with our Leica VT1200 vibratome. The component that holds the blade and vibrates (highlighted in the image) stopped functioning during sectioning. The blade...
02 December 2024 6,529 0 View
I am working on femtosecond laser drilling .The material on which i am performing the drilling processes is just 4 micrometer thick pasted on glass . Using Bessel beam our task to drill micro...
02 December 2024 2,406 0 View
Can someone explain to me without scientific mathematical "philosophy" why Nonlinear Feedback Shift Registers (NFSR) have not found wide application? After all, their cryptographic resistance is...
27 November 2024 5,577 0 View
My honorable/respected scholar friends please help me by giving simple method of removing the chlorophyll part from leaf extract without affecting the concentrations, denaturation, and structural...
25 November 2024 5,299 5 View
see in the video, it looks like bacteria to my eyes, but after a overnight incubation the medium is still pretty much clear. Got some Pen/Strep in the medium at 1%, incubated another night, media...
24 November 2024 2,123 1 View
I modeled a protein using AF3 and uploaded the structure to the PDBsum webserver alongwith my email ID. But I have not received any email with the analyes of the uploaded structure. Are others...
24 November 2024 7,890 2 View
Workforce management is a strategic process that focuses on planning, organizing, and optimizing an organization's human resources to meet its objectives efficiently. It involves activities such...
24 November 2024 157 4 View
Two-sided administrative leadership
01 November 2024 7,736 0 View
Hi all, I am having trouble deciding if this is cell contamination or something else. I keep seeing this fiber like bodies in my media (not on the same level as my adherent cells). There is no...
30 October 2024 9,026 1 View