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Questions related to Bioinformatic Tools
I am planning a study which will require long term humidity and temperature monitoring within 60+ tree canopies. I have identified the EasyLog USB Dataloggers as being the only affordable outdoor...
28 March 2023 7,706 1 View
Now I have obtained one Cre; Tdtomato mouse strain. How can I know whether this mouse strain is leaky or can be used?
10 February 2023 3,294 2 View
While docking using AutodockTools 1.5.7, this msg pops up in the command prompt while inserting protein, but grid parameter files and the binding affinity results are perfectly generated. Is it ok...
08 February 2023 5,135 1 View
Can anyone tell me the steps for grain yield stability analysis using the AMMI model for the incomplete block (augmented design) in Rstudio for a set of environments?
08 February 2023 1,567 0 View
Recent developments in AI shows the impact of language models on various disciplines. This will depend on how they are used and integrated into knowledge dissemination process.
03 February 2023 658 3 View
I am looking for the growth rate of the various parts of the strangler fig under best conditions including artificial irrigation with nutrient solution and a spot without shade. Location is GOA
20 January 2023 140 2 View
Hi there after i thawed my cells i encountered with this scene next days (even after i changed the media). there are dark plaques between my cells. they don't move or tremble and my media (DMEM)...
03 January 2023 1,985 0 View
I would like some advice regarding my experiment. I am using a transfection protocol to make viruses with my HEK293T producer cells. After I waited the required 48hrs, the produced virus...
25 December 2022 764 2 View
Dear researcher, I want to analyse the word /text cloud and want to create visualisations analytics that shows the most used words in a text form small to large. Information about free versions of...
19 December 2022 1,975 3 View
Hello everyone. I have data with three outcomes (two is binary and one is continous with poisson distribution) and around 10 independent variables. My objective is search for factors predicting...
15 December 2022 6,360 4 View
I'm new to the field, in a new lab, and looking for macrophage lines that would be best to use for some in vitro work involving adding tumor cells grown/ modified in vitro to macrophages. There...
02 December 2022 9,821 2 View
We are trying to detect variants at very low frequency in human tissues using WES (UMI-UDI libraries), aiming for a mean depth of 3000x, but we are getting very high duplication rates. In our...
28 November 2022 3,782 0 View
I am going to do sequencing for the first time and I am a bit confused how to count amount of chips that I need. I will use SMARTer smRNA-Seq Kit Takara for 96 reactions for library preparation....
08 November 2022 7,564 1 View
I made a journal, as an assignment for my course, but because I was still a beginner and had limited time, the journal still lacked a lot. do you have any suggestions on where to submit a journal...
03 November 2022 1,019 4 View
Dear Colleagues, I am working on the proximate analyses of leaf samples. I want a protocol to prepare leaf meal concentrate from leaf samples. I appreciate any...
03 November 2022 2,064 1 View
Hi, I recently thawed OVCAR-3 and after 6-7 days it looked like in the pictures. I wasn't sure if this was just cell debris or contamination. The medium wasn't cloudy but it was pink (like...
31 October 2022 9,167 1 View
I have started a docking study with 3t4p using AutoDock vina in UCSF chimera. The DWSI inhibitor interacts with residues such as Lys 51, Asn 82, Thr 83, and Ser 80. After redocking, DWSI...
29 October 2022 946 2 View
Hi everyone! I'm planning to do an in vitro macrophage infection with bacteria. I still do not know what kind of species I will be using, still haven't decided. those macrophages will be later...
28 October 2022 7,131 0 View
Hi everyone, I am searching for all PDB structures that have been solved for a specific protein. There are many homologs of this protein so I would like to narrow my search to structures that...
17 October 2022 8,806 3 View
In the description of a dataset in proteome exchange, one of the modifications is monohydroxylated residue. what is it? is it a fixed modification or variable modification?
30 August 2022 9,124 0 View
and what should the solution and amount be to block SUMO on yeasts? l found some thesis using ethanol, but haven’t seen how much should be add to the yeast. Many thanks!
19 August 2022 2,912 1 View
I am hoping to create a mock bacterial community and would like to ask for recommendations of commercial companies that offer customization services and can ship to Singapore?
14 July 2022 528 3 View
Hello, I am a student studying stem cell engineering. I am going to stain human stem cell-derived chondrocytes with Alcian Blue staining technique. I differentiated mesenchymal stem cells using...
13 July 2022 2,151 0 View
Due to the routine of finding new dysregulated genes from bioinformatics analysis, would it be possible to find genes which could be volunteers for vaccine design (for instance, multi-epitope...
04 July 2022 3,606 0 View