How to remove host DNA contamination from phage lysate?

More Baskaran Viswanathan's questions See All

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Interdendritic phase coarsens during homogenization?

Hello Is it possible for the micro-segregation (non-equilibrium phases) coarsen during the homogenization treatment? There is an obvious reduction in the quantity, but some of the phases remained...

19 September 2020 4,985 3 View

Could you please suggest me a good topic for my research paper (Tensor calculus with its applications in General relativity)??

i am currently working on topics in Tensor calculus with its applications in General relativity - im looking to write a research paper with whatever little background i have - Could you please...

18 September 2020 6,278 2 View

What could be the reason where i got some extra small peaks in my sample ?

Hi all, Actually, I have done the experiment where the single crystalline substrate has used to coat a thin film. In that, I have got substrate peaks and also thin-film material peaks also. But...

16 August 2020 6,532 11 View

How to apply a reversed cyclic loading in Midas FEA?

How to apply a prescribed reversed cyclic loading in Midas Fea? (Links for tutorials or snapshots of the steps will be a great help)

21 January 2020 2,471 1 View

How many hypotheses should I write for my study?

For my experimental study I created a course material for the visually challenged and implemented it by designing a learning model. Both are unique. Should I write two hypotheses, one for the...

25 March 2019 7,687 6 View

Site Directed Muatgenesis - Deletions not working! Please help ?

Hello I have been trying to make deletion constructs using site directed mutagenesis. The largest deletion is around 522 basepairs. My plasmid is around 11KB. I have tried inverse PCR primers,...

18 March 2019 4,591 5 View

Does Koops media supports the growth of Ammonia oxidizers alone?

Does koops media supports the growth of Ammonia oxidizing bacteria only? Or other heterotrophic microbes will also grow in the same media? Similarly Watson and Waterbury media supports only NOB...

18 March 2019 4,176 1 View

Dear All, I did Nyquist plots ac impedance measurements and I found the following plot. Can anyone help me to explain the observer trend with blank?

Dear All, I did Nyquist plots ac impedance measurements and I found the following plot. Can anyone help me to explain the observer trend with different concentration on steel with blank? Thanks...

24 February 2019 7,396 2 View

Where do I write about the Mnemonic Model I created and tested in an experimental study in the thesis? In Methodology section or a separate chapter?

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06 February 2019 4,391 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

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01 March 2021 3,355 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

Pierre Béguin

PEG precipitation followed by purification by CsCl density gradient centrifugation is the classic method for purifying phages. Nevertheless, there no such thing as zero contamination, only acceptable levels of contamination depending on the purpose for which the preparation is meant to be used.

Aryan Rahimi-Midani

Treat the lysate with DNAse prior to the DNA extraction.

Avtar Sain

use turbonuclease to remove host DNA.

Pradeep Kumar

add DNAse prior to the DNA extraction.