05 December 2024 1 6K Report

I have prepared an oil in water nano emulsion and would like to visualise it for confocal microscopy, however i dont have any fluorescent compounds in my emulsion. I know that nile red is a lipophillic dye and would like to know how to stain (protocol with concentrations) it so that i can visualise the droplet morphology better using confocal/fluorescence microscopy?

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Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

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What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

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10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

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Hello researchers Is this a random laser or just fluorescence?

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Do I need specific antibodies to stain for a fusion protein?

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Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

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How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

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Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

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Weak DAPI staining after immunohistochemistry - how to improve?

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05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

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04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

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Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

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