You can dilute directly in your tube, Maintaining the enzyme in neutral or buffer slightly acidic pH (e.g., pH 6.5-7.5) can help prevent oxidative damage. You can centrifuge your tube.

Maintain enzyme at low temperatures (such as 4°C or on ice) can slow down oxidative degradation, preserving enzyme activity.

For preserve or restore enzymatic axctivity you can use the reducing agent DTT (dithiothreitol) 10mM during 2 hours. Then remove the DTT by putting your enzyme in a dialysis membrane in your buffer.

Store in aliquots at -20°C. Avoid freezing or defrosting your enzyme too often.

Lyophilized proteins often stick to thLyophilized proteins often stick to the cap due to static charge. Here are some best practices we follow in our lab and recommend to our customers to minimize loss and safely recover the powder:

1. Centrifugation or Gentle Tapping

• Briefly spin the vial in a microcentrifuge or tap it gently to settle the powder at the bottom before opening.
• This helps bring the protein down from the cap and vial walls.
• Avoid vigorous shaking, as it may further disperse the powder.

2. Open in a Controlled Manner

• If the vial has a rubber stopper, carefully remove the metal seal while keeping the vial upright and steady.
• Open it inside a clean, dry biosafety cabinet or a draft-free area to prevent powder loss.

3. Reconstituting Directly

• If the protein is highly hygroscopic, consider dissolving it directly by carefully adding the buffer through the rubber stopper with a syringe and swirling gently.
• Allow it to sit for a few minutes to ensure full solubilization before transferring aliquots.

4. Aliquot & Store Properly

• Once reconstituted, prepare small aliquots to avoid repeated freeze-thaw cycles.
• Use low-protein binding tubes to minimize adsorption loss.
• Store at the recommended temperature based on stability data.

By following these steps, you should be able to recover most of your protein with minimal loss. I hope this helps! Let me know if you need further clarification.