Hey all, is someone using Drosophila files carrying a mutation in the fmr1 gene in particular the Delta50. All my stock are heterozygotes for the mutation being Tb... what do researcher for having them homo?
Microplastic
15 June 2024 5,416 2 View
An incorrect research has been added to my profile under my name and I wish to delete it.
10 December 2023 10,038 0 View
I've seen this term used in articles related to food web models, but I need a basic explanation for it. Thanks in advance.
23 August 2023 9,412 0 View
Dear colleagues, I am planning a project to characterize the plasmidome and virome of plants from metagenome and metatranscriptome sequencing data, however, I do not know what is the recommended...
04 June 2023 10,027 1 View
Hello to everyone. My work focus on studying PTMs and I have successfully phosphorylate my protein in vitro (Checking the reaction products by SDS-Page, all of my protein should be...
29 March 2023 1,640 3 View
I am studying the subcellular localization of a protein. To see if a GFP fusion protein can be compared to the native protein, I fused GFP to the N-terminal of the protein. In my western blots and...
01 March 2023 749 0 View
Hi I am trying to simulate a N-doped nanowire with two schottky contacts and the model wont converge for positive applied potentials, no matter how hard I tried. For negative applied potentials...
05 February 2023 1,624 0 View
Hi there, I want to perform different Machine Learning approaches for survival analysis in cancer. I have a database of 330 patients. I have some unique evaluated features so I can't use online...
25 December 2022 1,802 2 View
Hi there! I've just moved from ENDNOTE previous version to the last one v20. In the previous ones I could insert automatically a reference on while writing on word while being in the integraded...
14 March 2022 2,381 3 View
I have checked some collections (http://catalogue-cfbp.inra.fr/recherche.php http://www.wi.knaw.nl/collections/ https://www.dsmz.de/) but most of the strains were isolated a long time ago and the...
20 February 2022 2,781 1 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
I have done site-directed mutagenesis (Substitution) to obtain a Cysteine mutation in my gene of interest. Now, I have two domains in my protein, And I want to delete one of the domains to obtain...
05 June 2024 9,430 1 View
Now, I am using CRISPR/Cas 9 system to make a point mutation at THBD gene. I am wondering how can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are...
30 May 2024 9,993 0 View
We want to suppress self proteolysis of a protein. What is the strategy to modify the sequence in order to make it stable? The site is SLG - NX and SLG - AX.
25 May 2024 7,649 0 View
I am using GraphPad prism for data visualisation and analysis. I am trying to assess if there is a statistically significant difference between the percentage of 2 cohorts (patients and cell...
17 May 2024 4,424 1 View
I am working on a project to detect genetic mutations in lung cancer using machine learning algorithms and I am new to genetics and medical sciences. Can I use RNA as input to the algorithm for...
13 May 2024 5,726 6 View
This is from an endomyocardial biopsy from a 24 year old male with clinical hypertrophic cardiomyopathy. However, no mutation is found in the related genes. What are the irregular nuclear-like...
12 May 2024 8,148 1 View
presently I'm doing one study on ASSOCIATION OF COMT GENE VARIANT WITH ALCOHOL USE DISORDER. the methodology consists of conventional pcr with comt primers and rflp with NIA III rest enzyme. after...
22 April 2024 4,927 3 View
Dear all, I have performed CUT&TAG and tried to pulldown H3K27me3 containing nucleosomes in the cells treated with EZH2 inhibitor. I spiked my human cells with 20% of Drosophila cells. As...
21 April 2024 3,197 0 View