Antibodies for complex specific detection in a transferred Blue Native PAGE?

21 January 2025 0 8K Report

I am currently trying to optimise a method for Mitochondrial complex confirmation in Blue Native Page. I have transferred my gel to PVDF and incubated with OXPHOS antibody cocktail. I struggled to detect smaller complexes, and was wondering if anyone has recommendations for antibodies that are complex specific (I to V) that they have used in transferred Blue native pages in the past?

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