7h ago

Foudil Mohammed

If propolis extract literature is available then you have to select wavelength accordingly or if it is totally new work then you have to take a scan of solution by UV-Visible spectrophotometer in the range of 200nm to 800nm then you will get peak maxima. Select that wavelength for further analysis.

Feel free to ask if any doubt.

I found this information in an article :

Most flavonoids and phenolics show two absorption maximums at around 240–285 and 300–400 nm, which

correspond to the benzoyl and the cinnamoyl systems, respectively.

Due to the high molar absorptivity of the

different phenolic classes, 280 nm is the most generic wavelength for phenolic analysis using HPLC–UV.

For HPLC-UV analysis of propolis extract, optimal conditions include a C18 column with a mobile phase of 97.5% methanol and 2.5% acetonitrile at a flow rate of 0.8-1.0 mL/min, with detection at 290 nm for effective quantification of phenolic compounds.