I would like to have some more information for primer design for tRNA's, miRNAs and other sncRNAs.

I have used sRNAPrimerDB and mirPrimer ( for miRNA) before - are these reliable sources?

Any more reliable websites for designing primers?

Do you still follow the same rules as longer primer design ie checking melting temperatures and GC content ect?

Do you have to change your primer design depending on what assay you are dealing with - Taqman ( Stem loop) / probe/Sybr methods (mature miRNA)?

Any information you have on this will be helpful :)

Thanks

Danielle