How to Develop Optimized PARP Inhibitor Assays?

Amol Subhash Ingawale @Amol-Ingawale

08 July 2023 0 3K Report

-Various ways to measure PARP enzymatic activity

-How to optimize PARP assays

Badges
Science topic

Similar topics
Microbiology
Assays

More Amol Subhash Ingawale's questions See All

Modified graphical Representation of a data ?

Hello, If we generate a graph in some typical software and then without changing proportions or scale can we modify it? For example I created a graph of rarity of zooplanktons. Can I add picture...

14 April 2024 6,856 3 View

Whether Electromechanical SLIM type Control Relay will qualify "Seismic Shake Table Test" with SSE Vibrations ?

I have a Control System Panel having a PLC system and Control Relays installed in the panel. This panel is to be subjected to the "Seismic Shake Table Test with 5OBE + 1 SSE vibration level"....

27 March 2024 276 1 View

How to make the force field for cationic system such as benzene dimer cation?

Hello, Anyone can suggest me how to make the force field for small cationic system.

02 November 2023 3,590 2 View

Why does in a BOMD simulation, run multiple trajectories started from started from the same initial structure, exhibit exact identical trajectories?

When I perform Born-Oppenheimer Molecular Dynamics (BOMD) simulations of small organic clusters using the CP2K software in NVE condition, I notice that running multiple simulations from the same...

30 October 2023 5,134 3 View

How to calculate fraction of alpha helics of a specific region of protein in gmx?

I want to calculate the conversion of alpha-helics to coil shape of the specific part of protein. Please anyone can suggesting me how to do in gmx? Thanking You in Advance

29 July 2023 5,024 0 View

How can I create a trajectory from the generated trajectory in Gromacs that only includes solute and solvent molecules inside the cutoff of 0.35nm?

I want to generate a trajectory using my MD data that only contains solvent and solutes that are smaller than the 0.35 limit. I make the index file of those residue. From one frame to the next,...

05 July 2023 4,391 2 View

To make the amber or charmm force field of novel molecule?

Dear Researchers, I am doing molecular Dynamic simulation using GROMACS software. Currently i am working on the sugar modified double stranded DNA, for which i need to generate a force...

06 June 2023 3,957 2 View

Unknowingly my name was added as an author of a paper can i remove that now?

while creating my profile Unknowingly my name has been added s am an author of a peer I realized my mistake when I received a message regarding permission for full text. I am not an author of that...

12 May 2023 3,420 1 View

Need Information about where I can publish Bulk research paper/articles in different stream and areas?

Want to publish my No of research articles or research papers in a different stream or different area

27 April 2023 9,529 1 View

What does it mean when the periodic image distance is zero?

I calculated the periodic image distance of one strand in double helical DNA here, and it shows zero distance, which I don't understand. Could anyone please assist me?

08 February 2023 9,718 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View