-Various ways to measure PARP enzymatic activity
-How to optimize PARP assays
I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....
11 February 2021 6,558 2 View
I would like to know application of learning and control in MAS setting. And where could I find simulation codes of learning in MAS control
04 January 2021 1,866 4 View
Hi everyone, I am new gromacs user. I am doing the MD simulation of protein-NA. I did the Energy minimization step, NVT, NPT equilibration. after equilibrating the system i submitted the...
21 August 2020 7,876 4 View
I have MODIS hdf files for number of days. I want to extract the AOD data at a time for particular location.
09 July 2020 1,544 3 View
Hi, I working on Molecular Dynamics simulation of DNA. I want to study the 5'phosphate attached to thymine 16 number residue in my pdb file. I added these 5' Phosphate attached thymine in...
08 March 2020 8,195 1 View
Could anyone please provide a protocol for conjugation of antibodies to 30 nm gold nanoparticles?
20 November 2019 9,369 4 View
Dear researchers, I have some multiport extruded tubes with a certain white precipitate visible on the iner side of the tubes and would like to know a way to determine what that precipiate...
10 November 2019 3,318 7 View
without chamber for fatigue testing
24 January 2019 5,676 3 View
I am working on pressmud and its combinations for making good bio fertilizer for agriculture use.............
03 January 2019 2,756 4 View
Suggest thumb rules.
05 February 2018 3,578 4 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...
01 March 2021 6,474 3 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...
23 February 2021 7,429 1 View
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...
23 February 2021 9,933 1 View
New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...
21 February 2021 3,278 3 View
Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...
21 February 2021 7,397 1 View