I want to perform an antiviral assay for HIV inhibitors. I can't do the p-24 assay and I can only do the beta-galactosidase assay. So which cell lines can I use for this other than the tz-mbl cell?
You may use HELA-MAGI-CCR5 cells. The MAGI cell line (HeLa cells expressing an integrated copy of human CD4 and the HIV-1 long terminal repeat [LTR] fused to the β-galactosidase reporter gene) was engineered to express CCR-5. This cell line can be used to screen for anti-HIV drugs.
Please refer to the article attached below for more information on the cell line.
Dear Sarita Swain , in the past i made several experiments with HeLa-CD4-LTR-beta-gal cells (so called MAGI) or MAGIC5 cells ( MAGI-derived, CCR5-expressing cells ). Are there any details you could provide ? Good luck :)