Why the analytical method is better for evaluating the cloud? and what is the difference between simulation and analytical methods?

Hello every one, in several articles about"performance evaluation in cloud computing," authors announced that the analytical method is the most suitable for evaluating the cloud system, I want to...

22 October 2020 1,573 3 View

How we choose the most suitable analytical modeling technique for cloud computing?

Accurate performance evaluation of Cloud Computing resources is a necessary prerequisite for both client and provider to ensure that quality of service parameters remain within agreed limits. For...

15 October 2020 6,819 4 View

How can I show values of significance in a bargraph?

Hi, Please, is there anyone who knows how can I demonstrate p values (or asteriks of significance) on a bargraph. I need Free download softwares. Thank you.

21 February 2020 8,590 2 View

How to remove the solvent box from the em.pdb file after energy minimization in GROMACS?

Greetings all, I converted the .gro file to a .pdb file using the command: gmx editconf -f em.gro -o em.pdb To validate the minimized energy structure and calculate the RMSD using pymol, I need...

15 January 2020 9,151 4 View

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26 February 2019 2,499 4 View

How to model the free vibration of a beam loaded axially, either in tension or compression by Abacus software?

I am trying to model the free vibration of a simply supported beam with an axial loading to see the variation of eigenfrequencies when varying the axial loads.

30 April 2014 6,091 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View