I know that thermodynamically less stable premRNA molecules splice more efficiently but the consequences of this remains unclear for me.
what is the protocol for enzyme freeze drying?
09 July 2024 6,464 6 View
I don't have access to daily precipitation. Can I use monthly precipitation for running the HEC-HMS model? Let me know your expertise in Hec-HMS modeling. Thank you in advance for your kind help.
27 May 2024 1,495 1 View
I really appreciate if anyone can help
14 May 2024 1,959 1 View
In Silvaco, I have defined a semiconductor component. The electric field outputs have two problems. One is that the steps of X are not the same and they change, although I have defined a...
19 March 2024 4,649 0 View
Tissue culture and flowcytometry
16 March 2024 5,198 0 View
The study of personality traits has been a prominent area in psychology, and various theories and models have been proposed to understand and categorize these traits. What are these theories?
04 January 2024 1,288 5 View
Carbon Nanomaterials show enzyme-like characteristics (nanozymes), is there any research about nanozymes of carbon nano onions? Thanks
28 November 2023 8,227 2 View
Hello Can anyone help me estimate a New Keynesian DSGE model?
22 November 2023 3,018 0 View
by country by group
20 November 2023 9,441 1 View
Hi, At some point of the media, I observe some things, I don’t recognize this shape. Does anyone have an idea? Thank you for your help!
19 November 2023 8,185 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View