Which mobile phase, a wavelenght and a flow rate would be the best?
The column is packed with ODP stationary phase. My sample also contains NaOH, H2O2, therefore I need to avoid interference of these substances.
Dear Agnė Keselytė,
First of all, I would like to inform you that the best method for DMSO analysis is Gas Chromatograph (GC) because having solvent this technique is best suitable.
Gene@rally, DMSO is not analysis by HPLC because the traces can be remain in system and DMSO will cause corrosion.
"HPLC" is not an applicable scientific technique to use for the suggested compounds. Please use Gas Chromatography (GC) with a suitable detector and column for your application.
- With these types of basic questions, it is best to first research the topic to become acquainted with the applicable methods and techniques used. Please try a keyword search engine (e.g. Google or Bing) to find general information, example methods, application notes and journal articles to get started. Learning how to research a question is one of the most important skills you can learn.
Contact someone knowledgeable in GC analysis at your school for additional assistance. It takes many years of training and experience to learn just the basics of GC analysis (you can not learn it from taking a class).
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