Is there a paper that shows the effect of the thermal conductivity of solid electrolytes on lithium secondary batteries?

I want to know the effect of thermal conductivity characteristics of solid electrolytes on the all-solid battery, but I don't know which paper to refer to. Is there a paper on thermal behavior?

15 February 2021 8,806 2 View

Where can I buy Agilent software?

Hello. I have old agilent instrument, 6340 with 1100series HPLC, LC-Ion trap MS. We have software for LC, chemstation, but, not for MS. I asked Agilent korea if I can buy some software, and they...

05 January 2021 1,970 3 View

Another hyaluronic acid content analysis in cell culture?

Is hyaluronic acid content analysis by carbazole assay applicable to cell experiments? I'm looking for hyaluronic content analysis not use ELISA kit.

17 November 2020 6,990 2 View

How do I calculate the initial velocity from a progress curve?

I've done DTNB assay in order to measure HMG-CoA synthase activity. I know that I need to follow steady-state kinetics in order to apply Michaelis-menten equation, but the result confuses me a...

22 July 2020 2,520 10 View

What's the possible reason my brain(dentate gyrus) sections were damaged like this?

Dear all, I'm beginner of immunohistochemistry from dentate gyrus of mouse brain. As you seeing from the attachment, my brain sections seem to be damaged. 1. many holes 2. especially, tear of...

18 March 2020 2,375 7 View

How do I calculate solubility of a compound using a turbidity assay?

I'm planning to measure solubility of my compound in PBS buffer by measuring the turbidity of serially diluted solutions in a microplate reader at 600 nm. Can anyone please inform me how to...

23 November 2019 3,333 3 View

How can i scribble solar cell active layer for making electrode?

I fabricated solar cell by using flexible transparent conductive electrode(TCE). silver nanowire is deposited by solution process on the PC and PET. On the TCE, I coated ZnO ETL layer,...

01 August 2019 3,197 4 View

No title

29 October 2018 7,986 6 View

Length of locks in railway tunnel design in Germany?

Please let me know the reason of "locks of at least 12m long in railway tunnel design in Germany"? what is the reasoning of 12m long? Following is the original text in German: "In den...

25 February 2018 7,515 1 View

Is there any possibility of multiple transposon insertions into a chromosome of E. coli?

I learned that a mariner transposon requires only TA dinucleotides for transposition on some DNA strand. (no special sequence prefernce) Let's assume that a cut-and-paste mariner transposon exist...

18 January 2018 8,946 3 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Do I need to add a start codon and stop codon for the sgRNA expression in crispr?

Dear All, I am now designing the sgRNA for knocking out the gene through crispr. Base on my learning experience, a start codon and a stop codon should be put in the N terminus and the C terminus...

16 December 2020 5,795 3 View

How big can a enhancer deletion be ? how can we obtain homologous deletion clone ?

Hi all, I have tried to use Crispr Cas9 ( plasmid px333_cas9_mCherry) to delete some enhancers. I designed sgRNA using Chopchop website to target both sides of an enhancer to induce homologous...

13 December 2020 942 3 View

CRISPRa: ordering primers for NGS of amlified sgRNA library (PCR)?

Hello! I am performing genome-wide CRISPRa. I am following Joung et al. 2017 from Nature protocols. I have performed initial bacterial transformation of whole-genome and maxi prep. Now I need to...

17 November 2020 5,030 2 View

Where can I find off targets for Apis mellifera?

I would like to find off target sites for my sgRNA, however the more commonly used off target finders do not include apis meliifera, is there an application or website that would allow me to do...

09 November 2020 3,463 1 View

Why do I get a lot of colonies upon transformation of digested lenti guide puro?

I digested my lenti guide puro plasmid. The only modification in this plasmid is that it has blasticidin resistance gene instead of puromycin. I get the desired bands at ~8kb but I do not see the...

03 August 2020 2,854 4 View

Does this cold spring harbor protocol really edit circular dna with pcr?

Hello - I am studying this protocol which claims to use pcr to clone in/replace a small piece of an sgrna plasmid with a new guide sequence provided by an oligo with homology to the sgrna...

02 March 2020 6,358 2 View

Opinions on using PCR to re-target plasmid sgrna, with a "universal" sgrna scaffold?

I am using the following protocol from a CSH lab manual edited by Doudna on CRISPR, to attempt to retarget sgrna scaffold in a plasmid. but....I get DNA stuck in the well, not migrating....

01 February 2020 6,327 3 View

How do I rank CRISPR sgRNA pairs by on-target activity?

I am currently trying to generate CRISPR sgRNA pairs with Sanger Institute's online genome-editing tool. I enter the sequence of interest and it generates hundreds of sgRNA pairs, which I can rank...

09 October 2019 4,289 2 View