Does p-NitroPhenol acetate decomposes itself in Tris-HCl buffer without enzyme?

09 June 2023 0 7K Report

I was using para nitrophenol acetate for an esterase test in a microtiter plate. The reaction contained just crude enzyme, p-NPA, and also Tris-HCl buffer (pH8). The final product was p-NP(para nitrophenol) which gives yellow color and its OD is taken. Here, even in the blank(only buffer and substrate), the concentration of p-NP is coming in negative value.

What could be the possible reason?

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