Does anyone know how to remove a hardened agarose gel from an imager?

Our lab discovered a gel that was left overnight on our alphaimager with the UV light left on. It is now rock solid and very thin, fused to the surface of the imager.

01 February 2021 6,774 2 View

How to remove water from plant extract??

Hello everyone, I hope these words find you well. I used 80% methanol (solvent) for seaweed's extraction. I have performed rotary evaporator extraction in order to evaporate methanol but there is...

16 October 2020 5,598 8 View

How to dry nanoparticles without aggregating?

After freeze-drying my synthesized nanoparticles aggregate and do not disperse completely in water. Please help

05 October 2020 2,233 6 View

How to disperse freeze dried nanoparticles?

After freeze-drying, the nanoparticles could no longer be dispersed completely in water. Any suggestions on how to do it?

05 October 2020 7,814 4 View

How can we check the real content of calcium in the protein samples?

03 August 2020 4,309 3 View

Difference between systematic review and meta-analysis?

systematic review and meta-analysis

13 July 2020 4,041 18 View

Capability of MCNP6 in Muon tracking?

Is MCNP6 able to simulate galactic cosmic ray specifically Muons ? if yes does it have any energy or cross section restrictions?

16 June 2020 9,049 3 View

Can anyone provide or suggest some reliable articles on second order SEM modelling using SmartPLS.?

Hi everyone. I need some article to understand the second-order SEM modelling using PLS. If anyone can suggest or can share I shall be highly grateful.

03 March 2020 8,605 6 View

Help me construct a research topic on : open drug market and rising spate of drug abuse?

i need assistance on to construct a better research topic on the role of drug market on rising spate of drug abuse.

28 February 2020 8,251 3 View

Can I use an E-Beam patterned Photoresist as a mask for UV photoresist exposure?

Dear Seniors, I am wondering, if I could expose my pattern in E-beam resist (Suggest me one for this purpose) which is on top of UV photoresist (AZ some suitable one). Then I used this written...

21 February 2020 9,650 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View