Mammary glands in Platypus ?

Dear researchers, Can anyone help me to identify these odd mammary glands in Ornithorynchus anaticus ? I struggle to understand its mecanism : The secretion from all these flat lobes have a...

03 March 2020 8,275 2 View

Does anybody know any kind of preservation media for endometrial biopsy suitable for flow cytometry analysis?

I'm interested in any kind of preservation media for endometrial biopsy which could be stored at room temperature for at least up to 3 or 4 days. I've tested RNAlater media, but it seems to fix...

06 November 2018 1,845 2 View

What is the best DOE for finding an optimum compromise?

I am new and self taught in the area of DOE and after having read a lot of information on experimental designs I believe I have more doubts than right ideas. My goal is to find the optimum...

10 October 2018 2,094 4 View

Which is the best analytic column to separate antibodies and its variants?

I am looking for an analitic column to evalute the hidrophobicity profile of different antibodies and its variants. High resolution and high selectivity it is need, since am I interested in really...

10 September 2018 1,705 6 View

What's this peak?

Hi, i've injected 5 microliters of pure CHCl3 in a 250 mm C18 column, with ACN:H2O 80:20 as solvent, 1,0 ml/min. Something appears at 3,72 min, absorbing strongly at 210 nm. Is the CHCl3 absorbing...

20 December 2017 8,768 17 View

What do you think about this peak tailing?

21 March 2017 7,981 13 View

I have two insular populations, one of them have a low number of private alleles. This could be explained by an ancient bottleneck?

The neighbour populations belong to the same genetic pool, however they are morphologically differents, private alleles could be the explanation to an ancient selection of an adaptative shift?

27 July 2016 203 2 View

How to get dN/dS values for each branch?

I'm trying to test the selection model in an alignment of 40 coding nucleotide sequences, with its respective ML tree. I would need to get the dN/dS values for each branch of the tree but I'm...

24 December 2013 7,458 2 View

Does anybody know an automated method to quantify microglia?

Does anybody know an automated method to quantify microglia or has experience with the one described in the attached article? I would like to use it and would appreciate some...

25 September 2012 4,561 9 View

Do you plan to apply circular economy to use the energy produced inside the University or the neighbourhood?

Hello, I would like to know if you have some plan to use the energy locally. I've worked in different projects with Universities to move from fossil fuels to renewable energy to cover their...

01 January 1970 1,036 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View