I am currently performing DNA extraction for a transduced cell with a virus but whenever I perform PCR, there is presence of viral DNA in it. So may I know if adding DNase into the cell before extraction and performing washes will eventually eliminate viral DNA and not the transduced cell DNA?
You could use DNA extraction and purification kit for DNA extraction or you can simply use DNAse then wash the sample and use it for PCR. If you the result is okay then your problem is resolved. Good luck.
Farhana Mostofa Can I use DNAse to wash the cell first before performing the DNA extraction as it is able to remove other DNA contaminants?
First you have to protect your DNA of interest which you want to extract and then you can use DNAse to break down other DNA present in the sample. However, this is a bit tough to treat DNA like this.You can give a try if you find DNA purification ratio is ok then you can proceed on. Good luck.
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