What is the best concentration of Sudan black B to stain lipid granules in bacteria without leaves any dark particles in the slides?

07 June 2023 0 7K Report

I tried different concentration of Sudan black B (3 and 0.3%) in 70% of ethanol. I face the same issue when I stained my bacteria, the slide not clear and there are many of sudan black paticles in my slides make them bad. Even after filter Sudan black solution by 0.2um filter and using xylene to clean the slides to visualizing bacteria cell clearly

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