Absolutely. Fbs is full of growth factors, and depending on the cell line it may dramatically change the growth rate. Additionally, it will most likely affect gene and protein expression of cells as well, which will alter the phenotype of the cells. Overall, if you use higher fbs on cells, you will have to use higher percentage for all your experiments and all cell lines, or the data cannot be compared or interpreted.
Absolutely. Fbs is full of growth factors, and depending on the cell line it may dramatically change the growth rate. Additionally, it will most likely affect gene and protein expression of cells as well, which will alter the phenotype of the cells. Overall, if you use higher fbs on cells, you will have to use higher percentage for all your experiments and all cell lines, or the data cannot be compared or interpreted.
For sure it will affect and increase the growth rate as it is contains alot of growth factors.
You should expect to change your media pH really quick, let us know your observations!
FBS is importance in effecting to cell growth. Especially when you thaw cell frorm stock, you need to increase FBS percentage to help cell recovery!
your question depend of cell line.
Cell line is usually used at culture medium with a range between 5-10% FBS.
If you add less serum, you can generate oxidative stress. However when you add more serum, the effect of FBS on cell line ususally is not good or bad, but its use is recommendabled in some cases: frozen cellular, cell transport, etc...
This is an interesting question. As you increase serum from SFM conditions, then in many cells, this is beneficial, you see increased viability and proliferation, BUT it is not quite so simple. There is an optimal amount of serum that different cells like to grow and proliferate in, if you go above this concentration, then actually the excess serum is no longer beneficial and can actually become toxic - counterintuitive, perhaps? So when you say excess, what do you mean? If the optimal dose is 20% and you increase it to 40% - I suspect this will be detrimental to your cells, however, as I say an interesting question, so try it and see and let us know the outcome. Good luck.
Certainly an interesting question. You should be able to see some changes in the growth rate of the cells (you can determine there doubling time). FBS is rich in nutrients specifically high in albumin which can help nutrients entry in the cells. It is going to help your cells grow at faster rate. Also, if you are using cancer cell line, it will be really interesting to assess there growth rate in high glucose and high FBS environment. Please share your experience.
Usually the percentage of FBS will affect the growth rate of ur cells. The more you add, the faster it may grow. But, make sure your FBS is "heat-deactivated" before you add it to ur DMEM.
this is my question too...i used 40% fbs for thowing my cell because they 90% dead and i need that 10% and they were so important for me( i had just one stock remained)now i want to know can i trust to genetice background of my cells after this high dose of FBS!!?:(
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