Neetika Singh

11 Questions 13 Answers 0 Followers

Questions related from Neetika Singh

Analysing Docking results . Can someone help?

Hello, I am checking the anti- proliferative property of curcumin on cultured cells. I have docked my molecule with few receptors using discovery studio. I am not able to analyse my results. Can...

12 December 2016 4,457 0 View

Ecl not working?

I am doing western blot analysis for cultured cell histone. I am probing them with site specific modification antibody. To my dismay, i am getting result when I am developing the signal with DAB...

07 July 2016 4,139 2 View

Cultured cell histones not getting quantified ?

I am isolating histones using 0.4N H2SO4. I am using 1000000 cells and dissolving the desired histone pellet in 100 microlitres of Milli Q water with 0.1mM PMSF. Although I am getting good...

06 June 2016 9,687 5 View

What can I do when the media becomes turbid after 3 days?

I am working with HeLa cells and using DMEM supplemented with 10% FBS and 1X pen strep antibiotic as the culture medium. My cells grow well if they are revived from a cryoculture or even after...

05 May 2016 5,066 5 View

Should curcumin dilutions be prepared in cell culture medium or DMSO?

I am studying the anti cancerous effect of curcumin on cancerous cells (HeLa). I am dissolving the curcumin in DMSO and preparing the subsequent dilutions in DMSO. Is it a correct way or should I...

03 March 2016 2,330 2 View

Can curcumin be dissolved in 100% alcohol to check its efficacy as anti-tumor agent ?

I am studying effect of curcumin on HeLa cells as an anti-tumor agent. I am dissolving it in 100% alcohol. Should I be using some other solvent?

01 January 2015 5,657 4 View

Can anyone help with histone isolation from Hela cells?

I am trying to isolate histones from Hela cells but my preparation is not coming out clean. I am getting other contaminants along with the preparation. Can anyone please come up with suggestion?

07 July 2014 5,539 3 View

Is there any way to quantify the cleavage of a protein?

Normally protein cleavage can be determined qualitatively on an SDS PAAG. Is there any way to quantify the same qualitative observation in terms of numbers?

05 May 2014 285 5 View

What if FBS is added in excess in DMEM cell culture media?

Can it affect the growth of cell?

01 January 2014 9,423 12 View

Cell culture - can anyone help?

I am setting up cell culture facility in my lab. Initially, I am taking off with HeLa cells. I am ready with media and all other preparations. As a part of scientific community, I hereby ask...

10 October 2013 7,064 9 View

My PCR product is non specific. What can be the probable reasons for the same?

The set of primers are well designed with the sequence and even after doing Primer Blast , they are showing only one specific amplification .

09 September 2013 5,239 6 View