49 Questions 91 Answers 0 Followers
Questions related from Abbas Raza
Hi there I've a congenic strain of mice that exhibits a phenotype dependent on testis (lost following orchiectomy) and was wondering if i can assess the contribution of Testosterone or DHT using a...
10 October 2018 2,468 2 View
Hi there I am analyzing SNPs from two different datasets that once collated will provide a complete picture of haplotype blocks. The issue I am facing here is there are SNPs that are found in both...
07 July 2016 8,186 1 View
I am looking at SNPs between different strains of mice for a particular interval. When I try to use the MGI for SNP analysis http://www.informatics.jax.org/ I find strains that have multiple data...
07 July 2016 4,082 1 View
Hi there I've a total of 8000 list of genomic coordinates from mouse that I want to convert to gene names, is there a software out there that can do it quickly rather than I doing them manually...
07 July 2016 3,958 6 View
Hi, i am planning to measure some cytokines in mouse serum at different time points in my model and was wondering if anyone has done these retro-orbital blood collection and the amount that one...
09 September 2015 7,766 3 View
Hi there, i am looking for some guidelines to using silastic tubing for implanting hormones in mice. I need to provide 50ug of drug released every day. Has anyone worked with them and would be...
06 June 2015 2,457 5 View
Hi there, i am looking for the appropriate dosage of DHT in C57BL/6 mice that can be given orally to compensate for gdx. I haven't found an appropriate study that has used it. If any investigator...
06 June 2015 8,362 5 View
I am looking for an explanation regarding ubiquitin-proteosome-system of protein degradation: 1. How is ubiquitin itself turned over? 2. Why proteosome don't break peptide bonds in ubiquitn when...
04 April 2015 531 0 View
I am working with histamine receptor 1, a G protein couple receptor (GPCR), and when i prep my lysate for SDS page gel run I heat them up at 95C/3 min. I am getting protein aggregation in my...
03 March 2015 4,304 6 View
I was analyzing CNS cells for any infiltration of T cells in one of our disease models and found dual staining of TCRgd and TCRb, has anyone found this or maybe its an articraft? any thoughts?
02 February 2015 8,240 7 View
Has anyone come across using S-myc tag rather than the conventional c-myc protein tag? I received a construct that had S-myc and I am not sure if this is the same tag as c-myc. There is no...
02 February 2015 10,085 6 View
I was wondering if anyone has come across membrane lipids that have 3 or more hydrophobic tails. I was thinking along the oxidised phospholipids in disease that might incorporate more tails but...
01 January 2015 4,430 6 View
Hi, I am interested to isolate mouse liver and heart for my experiment. Does anyone know of a good video resource for the procedure? I searched on jove.com but couldn't find the surgical...
12 December 2014 2,495 3 View
I want to know if anyone has made a N-terminal tagged FLAG expression construct of histamine receptor 1. I wish to express the full length protein but wondering if I need an extra linker between...
11 November 2014 7,901 4 View
I am looking for a an explanation about drug potency and how it relates to the constant Ki of that drug? Are they proportional? Is it possible for a drug to be potent yet have high Ki?
10 October 2014 3,982 12 View
Has anyone used the specific Histamine receptor 1 agonist 2-Pyridylethylamine dihydrochloride in mice and what dosage? I haven't found any literature using this drug in a mouse model. Any help?
10 October 2014 8,283 2 View
Does anyone know a good place to find the absorption spectra and peak wavelength for photoreceptors proteins? I am interested to find out the absorption wavelengths for photoreceptors in archaea...
10 October 2014 6,220 0 View
I used to use pDRAW for my TA class however it has some issues with installation and crashes with different windows versions. I am looking for an alternate genome annotating software that I can...
09 September 2014 8,764 7 View
pKa of acetic acid is 4.75 whereas of glycine is 2.93. So whey is it more acidic? Simarly, why is the amino group "less" basic (9.6 vs 10.6) please provide an easier to understand explanation
09 September 2014 5,142 11 View
i was wondering if it is to do with the phi and psi angles of tryptophan that doesn't make it favorable to be found in proteins or is it to with its high metabolic expense that it's not so common...
09 September 2014 7,945 10 View
Any suggestion or paper guidance will be appreciated.
09 September 2014 1,550 4 View
I was wondering if anyone has tried making blocking solution (5% donkey serum) in PBST and also in PBS and looked at immunostaining results. From my western blot knowledge we don't make blocking...
08 August 2014 6,635 7 View
Is water ionization different for both or not?
08 August 2014 2,288 3 View
I am working on identifying phosphorylation site (s) within a novel protein and and their roles. It appears through mass spectrometer of my protein that there are phosphorylated serines,...
07 July 2014 7,787 5 View
I don't have an idea about mass specs of proteins but am curious to know if it is possible to have a relative abundance of phosphorylated/unphosphorylated protein from mass spec data?
07 July 2014 1,458 6 View
Proteins in cell are continually being degraded in proteosome machinery and loaded on MHC's. I was wondering if these peptides or self antigens are displayed and if yes how are they recycled if...
07 July 2014 2,291 9 View
I am having a tough time figuring out problems in my PCR where I am using a set of mouse IL2 primers and amplifying a segment. I am getting amplification with no template as well. I changed all...
05 May 2014 4,185 22 View
I am looking for a physical explanation on the fact that if prokaryotes had some kind of endomembranes it would not have been feasible for them to perform their functions. Any suggestions on this...
05 May 2014 3,163 2 View
There are many ways through which enzymes increase their rate constants and one of them is called as encounter complex. I am looking for an explanation of this process. Any help or figure would be...
05 May 2014 3,743 1 View
Has anyone used the SCOP website? I tried to search for my protein pdb id 3w0f but could not get its classification. Does anybody know how to get around this problem? The website is very user...
05 May 2014 7,028 5 View
I am looking to work on an experiment and need some information about cell population of mast cells in lymph nodes and spleen. which one has more?
05 May 2014 8,548 3 View
I had a confusion regarding tag buffers when I was running my PCR reaction and I accidentally pulled out a vial of MgSO4 as opposed to MgCl2. My PCR ran blank and I am kind of wondering if it has...
05 May 2014 9,127 5 View
Can anyone give me a good explanation or annotated figure of where this technique is used to knock in any gene at embryonic stage?
04 April 2014 1,403 5 View
I would like to to know the cell types expressing these.
04 April 2014 6,784 19 View
I came across a figure and was wondering which cells are sorted by CD4-/CD8- cells.
04 April 2014 7,259 4 View
I am looking for an easy to search promoter database where I can look up promoter sequences for mouse genes rather than going to NCBI and taking the long and painful journey searching for my gene....
04 April 2014 7,580 3 View
I am curious to know if there is still a debate going on regarding the coverage of human genome (around 80% coding) as concluded by the ENCODE project papers or still we have opponents who believe...
03 March 2014 6,925 7 View
I would appreciate any comments as to which loading control is generally used for cytoplasmic lysates: alpha actin or beta actin. What are the reasons for why you prefer the one over the other?
03 March 2014 4,467 3 View
I am curious about where to submit my manuscript. If I go with how well my work fits with specialized journals in the field I end up losing my chances for the big names. But do these big names or...
03 March 2014 9,206 7 View
Looking at the incorporation of selenium in proteins like glutathione peroxidases, thioredoxin reductases, formate dehydrogenases, and 20 other proteins it is inquisitive to find out if increased...
03 March 2014 6,326 3 View
Either 3'RACE or 5'RACE.
03 March 2014 777 10 View
I was going through a couple of studies where the spectrum of cancer cells within a single biopsy site were shown to be heterogeneous having different expression of genes and showing non-Darwinian...
03 March 2014 3,088 11 View
I was wondering if somebody can guide me regarding how representative cDNA libraries are e.g. do they actually show just 5% of full transcriptome or do we have libraries that show more coverage?
02 February 2014 7,059 2 View
I am having difficulty understanding the theory behind cloning used back in 80's when we didn't have access to the full human genome. I was wondering if somebody could explain to me the...
02 February 2014 2,217 3 View
I am looking for a good explanation of the process, namely the molecular biology of this process. Can someone suggest an annotated diagram or paper figure?
01 January 2014 1,905 3 View
My background is related to proteomics so a beginner's guide to crystallography or something would be good.
01 January 2014 7,684 13 View
On DNA agarose gels we take the position of these two dyes as approx 3500 bp (xylene cyanol) and 300 bp (bromophenol blue). I am curious to know the mobility values (in bps) of these two dyes when...
08 August 2013 6,576 5 View
We normally perform in-vitro transcription using nuclear extracts, rNTPS and template DNA and detect the species synthesised. Can somebody tell me how would in-vivo transcription compare if the...
08 August 2013 5,598 0 View
I am interested in the role of invariant Natural Killer T cells (iNKT) in the initial response to HBV infection inside hepatocytes. I have seen this cell line used in many such studies so I...
07 July 2013 4,079 2 View
