Jhoti Somanah

16 Questions 35 Answers 0 Followers

Questions related from Jhoti Somanah

What kind of programmes/courses does the Health Science/Bioscience dept. run in your institution?

We're a newly set up department (Health Sciences), I'm brainstorming for some potential programmes/courses that we could run. Our team consists of MBBS doctors and PhD holders who have experience...

08 August 2016 2,520 0 View

Does anybody have any experience with using comet assays?

Have some basic queries regarding initial setup: 1) Do I need to use the microscope in fluorescence mode? 2) If I put my stained slide on the stage, will the image on the screen remain black, or...

10 October 2014 2,932 6 View

Can I preserve my COMET assay slides?

Hi, I'm doing the Alkaline COMET assay. But since I have many samples I wanted to know if it is possible to preserve my slides after doing the electrophoresis? I want to preserve the slides, so...

08 August 2014 5,130 18 View

Does anyone have experience with a Leica DM IL microscope- How does one use fluorescence?

We have a Leica DM IL inverted microscope. Can anyone help me out on how to switch to the fluorescence settings? When viewing my stained slides.. Instead of a black background, I am viewing a grey...

08 August 2014 6,016 3 View

Why is my TMB substrate turning blue before I add it to my wells?

Im doing the ELISA assay, but my TMB substrate is turning pale blue BEFORE i'm adding it to my wells (whilst mixing). Is this a reaction due to exposure to light? How can i avoid this color change?

07 July 2014 6,046 26 View

What does cell culture supernatant mean?

I want to determine TNF-alpha levels in my cell culture. The protocol mentions that i should use Cell Culture Supernatant for this test. There is no mention for the use of any lysis buffer. So...

06 June 2014 7,054 13 View

How do I prepare cell culture lysate for an ELISA?

These questions may sound silly, but this is all quite new to me! I have two queries: 1. If I grow my cells in 24/6-well plates, is it safe to trypsinize the cells first then lyse in buffer? Or...

05 May 2014 2,087 6 View

What can I add in my CO2 incubator water reservoir to prevent fungal/bacterial growth?

Usually I add some copper sulphate to my (filtered) autoclaved distilled water in the water reservoir pan, but something orange-white (fungus?) keeps appearing in the water. What else can i add...

04 April 2014 4,568 23 View

My cell cultures were exposed for 3 hours to some (very little) sodium azide in the incubator water, will this affect my cells?

Do sodium azide fumes react with cells immediately? I quickly replaced the water with fresh autoclaved, but I'm worried that this short exposure (3hrs) may affect my cells. Please advise!

04 April 2014 4,173 0 View

I need to measure ROS content in my cells using 2' ,7' - dichlorofluorescein diacetate (DCFH-DA). Should this be diluted in DMEM or in DMSO?

04 April 2014 1,364 6 View

What are the best antifungal agents I can use for my cell culture?

I'm currently using amphotericin B, but it seems the fungus is resistant to this. Has anyone used nystatin, clotrimazole, or econazole? If yes, at what dosage? I'm using fibroblast cells.

03 March 2014 7,331 9 View

Does dead bacteria sink or float in its growth medium?

I'm doing a biofilm assay with streptococcus mitis and mutans. I get excellent biofilm formation after 48-72hr hours incubation (rather than standard 24Hr), but i am worried that this biofilm...

03 March 2014 1,997 8 View

For cytotoxicity testing, should I pre-treat my cells with extract for 24 hours or 48 hours?

Would you recommend treating cells with a plant extract for 24 hours or 48 hours?

02 February 2014 2,770 4 View

Is it possible to use an autoclavable dish filled with sterile water and CuSO4 in a CO2 incubator?

I'm frequently experiencing contamination of my cell cultures and strongly suspect it to be due to the water in my CO2 incubator's reservoir. The incubator is quite big, so it is difficult to...

02 February 2014 3,143 1 View

My fibroblast cells are growing very slowly, what can I do to accelerate their growth?

I'm using DMEM with glutamine and 10% FBS. Since a month now, the growth rate has seriously dropped. No contamination has been detected. What can I do to make them grow quicker?

02 February 2014 1,669 4 View

What is the recommended incubation time for my cells to be treated with extract (for MTT assay)?

I need to pre-treat my cells with a plant extract first. What is the recommended incubation time for my cells to be exposed to my extract for this MTT assay- 24 hours or 48 hours? I'm using...

02 February 2014 412 6 View