Analysing Docking results . Can someone help?

Hello, I am checking the anti- proliferative property of curcumin on cultured cells. I have docked my molecule with few receptors using discovery studio. I am not able to analyse my results. Can...

11 December 2016 4,396 0 View

Ecl not working?

06 July 2016 4,103 2 View

Cultured cell histones not getting quantified ?

I am isolating histones using 0.4N H2SO4. I am using 1000000 cells and dissolving the desired histone pellet in 100 microlitres of Milli Q water with 0.1mM PMSF. Although I am getting good...

05 June 2016 9,656 5 View

What can I do when the media becomes turbid after 3 days?

I am working with HeLa cells and using DMEM supplemented with 10% FBS and 1X pen strep antibiotic as the culture medium. My cells grow well if they are revived from a cryoculture or even after...

04 May 2016 5,021 5 View

Should curcumin dilutions be prepared in cell culture medium or DMSO?

I am studying the anti cancerous effect of curcumin on cancerous cells (HeLa). I am dissolving the curcumin in DMSO and preparing the subsequent dilutions in DMSO. Is it a correct way or should I...

02 March 2016 2,290 2 View

Can anyone help with histone isolation from Hela cells?

I am trying to isolate histones from Hela cells but my preparation is not coming out clean. I am getting other contaminants along with the preparation. Can anyone please come up with suggestion?

06 July 2014 5,498 3 View

Is there any way to quantify the cleavage of a protein?

Normally protein cleavage can be determined qualitatively on an SDS PAAG. Is there any way to quantify the same qualitative observation in terms of numbers?

04 May 2014 213 5 View

What if FBS is added in excess in DMEM cell culture media?

Can it affect the growth of cell?

31 December 2013 9,344 12 View

Cell culture - can anyone help?

I am setting up cell culture facility in my lab. Initially, I am taking off with HeLa cells. I am ready with media and all other preparations. As a part of scientific community, I hereby ask...

09 October 2013 7,022 9 View

My PCR product is non specific. What can be the probable reasons for the same?

The set of primers are well designed with the sequence and even after doing Primer Blast , they are showing only one specific amplification .

08 September 2013 5,195 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View