14 Questions 61 Answers 0 Followers
Questions related from Bashir Alsiddig Yousef
We use to use FBS form Gibco to culture our colon cance cell lines (HCT-116, COLO-205, HT-29 and SW-620) and it were o.k, with good morphological appearance as in the database, I sub-cultured them...
11 November 2014 8,056 6 View
I am working in natural compound that has anticancer activity against Colorectal Carcinoma, I want to study the effect of this compound on receptor expression and activation, so I want to know if...
10 October 2014 2,505 1 View
I am intending to use TGF-beta receptor 1 antibody as a blocker in cultured fibroblasts treated with TGF-b. In general, to assess the FMT (fibrobllast-to-myofibroblast transition), cells are...
06 June 2014 9,382 2 View
Sample denaturation is one of the steps that should be carried to prepare the proteins for SDS-PAGE. Should we carry this step out for all proteins? Or can some proteins be affected by this...
03 March 2014 7,519 14 View
I am targeting NF-kB pathway in cancer cells, and my drug may block this pathway. According to my hypothesis, I will use western blot and qRT-PCR to confirm this effect. Should I use a positive...
02 February 2014 7,046 10 View
I am working on a drug that is supposed to block NF-kB, so I want to study the effect of the drug on protein and mRNA expression in colorectal cancer cells. Should I induce NF-kB in these cell...
01 January 2014 5,276 7 View
I am planning to check antiangiogenic activity for my compound, but I don't know what cells I should use to study the in-vitro and in-vivo antiangiogenic activity of this compound?
06 June 2013 3,768 4 View
When we want to carry flow cytometry for cell cycle analysis or for apoptosis study as examples, we have to use Trypsin to harvest the cell without EDTA. Why do we have to use Trypsin without...
06 June 2013 5,799 2 View
I am using culture media to dissolve my drug as the drug is not stable in DMSO, so I am keeping fresh drug solution for when I want to carry any experiment (cytotoxicity, cell cycle analysis,...
05 May 2013 5,125 9 View
I am doing a cytotoxicity study for a group of drugs in different cell lines using an MTT assay. I am wondering about cell passage (Subculturing). One time I worked in an early passage (such as...
05 May 2013 1,905 6 View
I have worked in NCI-H1650 (human non-small cell lung cancer cells), I have thawed these cells for 3 times, in all the cells were ok and attached well after 24hr, but after 48hr the cell is dying...
03 March 2013 8,249 5 View
If I want to freeze the cells, should I wait for a certain number of subculturing? Or can I do it after just one passage of cell?
03 March 2013 8,757 7 View
What happens if we increase the concentration of antibiotic in a medium to 2%, as an example? Is it harmful for the cell? Or can it affect the cell as these antibiotics work selectively on bacteria?
03 March 2013 8,951 12 View
I used to use ready antibiotic supplied by a company, now I need to prepare antibiotic solution for cell culture purpose from streptomycin and ampicillin powder. What exactly do I have to do to...
02 February 2013 7,133 9 View
