Hello,
I am checking the anti- proliferative property of curcumin on cultured cells. I have docked my molecule with few receptors using discovery studio. I am not able to analyse my results. Can someone suggest me something regarding the same.
I am doing western blot analysis for cultured cell histone. I am probing them with site specific modification antibody. To my dismay, i am getting result when I am developing the signal with DAB...
06 July 2016 4,156 2 View
I am isolating histones using 0.4N H2SO4. I am using 1000000 cells and dissolving the desired histone pellet in 100 microlitres of Milli Q water with 0.1mM PMSF. Although I am getting good...
05 June 2016 9,706 5 View
I am working with HeLa cells and using DMEM supplemented with 10% FBS and 1X pen strep antibiotic as the culture medium. My cells grow well if they are revived from a cryoculture or even after...
04 May 2016 5,083 5 View
I am studying the anti cancerous effect of curcumin on cancerous cells (HeLa). I am dissolving the curcumin in DMSO and preparing the subsequent dilutions in DMSO. Is it a correct way or should I...
02 March 2016 2,347 2 View
I am studying effect of curcumin on HeLa cells as an anti-tumor agent. I am dissolving it in 100% alcohol. Should I be using some other solvent?
31 December 2014 5,683 4 View
I am trying to isolate histones from Hela cells but my preparation is not coming out clean. I am getting other contaminants along with the preparation. Can anyone please come up with suggestion?
06 July 2014 5,564 3 View
Normally protein cleavage can be determined qualitatively on an SDS PAAG. Is there any way to quantify the same qualitative observation in terms of numbers?
04 May 2014 315 5 View
Can it affect the growth of cell?
31 December 2013 9,460 12 View
I am setting up cell culture facility in my lab. Initially, I am taking off with HeLa cells. I am ready with media and all other preparations. As a part of scientific community, I hereby ask...
09 October 2013 7,083 9 View
The set of primers are well designed with the sequence and even after doing Primer Blast , they are showing only one specific amplification .
08 September 2013 5,259 6 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View