Ecl not working?

06 July 2016 4,103 2 View

Cultured cell histones not getting quantified ?

I am isolating histones using 0.4N H2SO4. I am using 1000000 cells and dissolving the desired histone pellet in 100 microlitres of Milli Q water with 0.1mM PMSF. Although I am getting good...

05 June 2016 9,656 5 View

What can I do when the media becomes turbid after 3 days?

I am working with HeLa cells and using DMEM supplemented with 10% FBS and 1X pen strep antibiotic as the culture medium. My cells grow well if they are revived from a cryoculture or even after...

04 May 2016 5,021 5 View

Should curcumin dilutions be prepared in cell culture medium or DMSO?

I am studying the anti cancerous effect of curcumin on cancerous cells (HeLa). I am dissolving the curcumin in DMSO and preparing the subsequent dilutions in DMSO. Is it a correct way or should I...

02 March 2016 2,290 2 View

Can curcumin be dissolved in 100% alcohol to check its efficacy as anti-tumor agent ?

I am studying effect of curcumin on HeLa cells as an anti-tumor agent. I am dissolving it in 100% alcohol. Should I be using some other solvent?

31 December 2014 5,611 4 View

Can anyone help with histone isolation from Hela cells?

I am trying to isolate histones from Hela cells but my preparation is not coming out clean. I am getting other contaminants along with the preparation. Can anyone please come up with suggestion?

06 July 2014 5,498 3 View

Is there any way to quantify the cleavage of a protein?

Normally protein cleavage can be determined qualitatively on an SDS PAAG. Is there any way to quantify the same qualitative observation in terms of numbers?

04 May 2014 213 5 View

What if FBS is added in excess in DMEM cell culture media?

Can it affect the growth of cell?

31 December 2013 9,344 12 View

Cell culture - can anyone help?

I am setting up cell culture facility in my lab. Initially, I am taking off with HeLa cells. I am ready with media and all other preparations. As a part of scientific community, I hereby ask...

09 October 2013 7,022 9 View

My PCR product is non specific. What can be the probable reasons for the same?

The set of primers are well designed with the sequence and even after doing Primer Blast , they are showing only one specific amplification .

08 September 2013 5,195 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

No title

28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View