I am working with HeLa cells and using DMEM supplemented with 10% FBS and 1X pen strep antibiotic as the culture medium.

My cells grow well if they are revived from a cryoculture or even after splitting in a ratio of 1:1 but if I grow them at a lower seeding density and keep them for 3 days under observation or for experiment purpose, instead of growing as a confluent monolayer, my cells start dying and there is a stinky smell coming form media. Moreover, the color of also media becomes turbid 

Kindly help.

More Neetika Singh's questions See All
Similar questions and discussions